Prof.Dr.
Near East University, Obstetrics and Gynaecology; veterinary Faculty
Englisch: Curriculum Vitae Name Prof.Dr. Selim ASLAN Official address Near East University Veterinary Faculty Clinic for Obstetrics and Gynaecology Nicosia-TRNC selim.aslan@neu.edu.tr Tel:+3926751000/3145-3150 Marital status married Nationality Turkey Place and Date of birth Turkey, 26.12.1956 Education/Degrees: 2000- :Professor : University of Ankara 1995- : Associate Professor 1987-199 : PhD (Veterinary University Vienna/Austria) and Research (Advisor: ProfDDr. Kurt Arbeiter) Professional Appointments: 1983-1984 Continuing Education at the clinic in Bad-Hersfeld (Germany) 1985-1987 Research Assistant (Faculty of Veterinary Medicin –Ankara) 1987-1992: PhD (Veterinary University Vienna/Austria) and Research 1992-1995: Ankara University Veterinary Faculty. Clinic of Obstetrics and Gynaecology: Dr. Assistant /Ass.Prof. (1993) 01.03.1997-31.08.97 University of Veterinary Medicine Vienna: Clinic of Obstetrics, Gynecology and Andrology (Visiting Professor), 1998-2011 Ankara University, Faculty of Veterinary Medicine, Prof. Dr. 1999: Presentation at the Symposium "Small Animal Reproduction" on the occasion of the 70th birthday of em. Prof. Dr. DDr. h. c. Kurt Arbeiter: “Termination the early pregnanch in the Bitch” 2000- Munich, Maximillian University, Veterinary Faculty (Prof. Stolla) DAAD Scholarship (Research: Blood flow in the corpus luteum in cattle) 2001: Invitation of the Association of Veterinarians for a lecture in Tirol . Reproduction and Neonatology Congress, Alpbach, Tirol. 2008: Hannover, Clinic for Cattle, School of Veterinary Medicine (Prof.Dr. H. Bollwein) DAAD scholarship (Research: Effects of induction of ovulation with GnRH and hCG on follicular and luteal blood flow in Holstein Friesian heifers) 2011-……. Senior Scientist , Project Collaboration : Turkey (with various faculties together) , Vienna, Switzerland Clinic of Obstetrics, Gyneacology/ Institute for Anatomy and Histology (Senior Scientist,Project Collaboration) 2013: Invitation of the University of Zurich (Institute of Reproductive and molecular histology and anatomy on): Lecture: Introduction abortion in the bitch: Selecting the right method (Project Preparation and Presentation) Research and Clinical Fellowships: 1995-1996: Research and Clinical Work at the Veterinary University Vienna; Lectures at the Vet Med Ankara 1989- until to day : Participation in the fertility - and sterility congress (Februartagung) in Giessen, Munich, Berlin, Hannover, Leipzig and Zurich Current research Luteal Blood Flow and Luteal Size in the Cow causes of poor fertility in high milk producing dairy cows Beneficial effects of Beta-carotene injections on the fertility of postpartum dairy cows Contraception in dogs and cats (Induction of abortion; Pregnancy termination) Immunology of pregnancy and parturition (Expression of genes in the canine uterus and embryo; Immunological aspects during early canine pregnancy; Expression of genes in the canine preimplantation uterus and embryo:implications for an active role of the embryo before and duringinvasion; Embryo–maternal interaction in early-pregnancy Heard Management in the Cow Scientific Cooperation: Akıllı Sürü ve Çiftlik Yönetim Karar destek Sistemi) (Smart Herd and Farm Management Decision Support System), Tübitak TEYDEB Project; 1501; Veterinary University of Vienna, Clinic for Obstetrics and Gynecology Oktober 1987 –1998 (Prof.DDr. K. Arbeiter); 2000- until today we have projects together (Prof.Dr. Sabine Shaefer-Somi) Clinic for Reproduction , Vetuisse-Faculty, University of Zurich, Switzerland (Prof.Dr. Heinrich Bollwein) 2000-until today we have projects together Justus-Liebig-Universität Giessen, Veterinary Medicine, Clinic of Obstetrics, Gynecology and Andrology; Germany (Prof.Dr. B. Hoffmann ) 2005-2011 Institute of Veterinary Anatomy, Vetsuisse-Faculty, University of Zurich, 8057 Zurich, Switzerland 2006- until today we have projects together (Prof.Dr. Mariusz Kowalewski ) Department of Large Animal Sciences, Section Veterinary Reproduction and Obstetrics, Denmark, 2014- (Ass.Prof.Dr. Sandra Goericke-Pesch) Grants / Funds / Promotions - Third party funds by Virbac Austria GmbH Research promotion for the investigation of repeated induction of abortion with the antiprogesteron Aglepristone, and for the use of the GnRH Analogue Deslorelin in prepubertal dogs (material expenses). - Grant of the Uni Ankara (research promotion; In different times) - Research promotion of the Vetmeduni Ankara (Expression and activity of matrixmetalloproteinases in the uterus of bitches after spontaneous and induced abortion; - Third party funds by Virbac Austria GmbH Research promotion for the investigation of repeated induction of abortion with the antiprogesteron Aglepristone, and for the use of the GnRH Analogue Deslorelin in prepubertal dogs (material expenses). Alvetra&Werfft AG, Austria : The change in luteal blood flow and luteal size after the beta carotene injections in early pregnant dairy cows Memberships Austrian Society of Veterinarians (ÖGT) European Society for Domestic Animal Reproduction (ESDAR) Society of Homöpaty (Honor President) Deutsch: LEBENSLAUF Persönliche Daten: Vorname: Selim Familienname: Aslan Akademischer Titel: Prof. Akademischer Grad: Dr. med. vet. Geburtsdatum: 26.12.1956 Geburtsort: Iskenderun, Türkei Arbeitsplatz: Near East University, Clinic for Obstetrics and Gynaecology Nicosia-TRNC-Mersin 10 selim.aslan@neu.tr z.Z. Wissenschaftliche Arbeit an der Veterinärmedizinischen (Senior Scientist): Universität Wien;Prof.Dr. Sabine Shaefer-Somi), Zurich (Prof.Dr. Mariusz Kowalewski; Anatomie und Hystologie) , Zürich (Prof.Dr. Heinrich Bollwein; Rinderklinik); Denemark (Ass.Prof.Sandra Goericke Pesch) Familienstand: verheiratet, 1 Kind Kontaktadresse: Elisen straße 6/4 A-1023 Wien Aufenthaltsstatus Österreich: unbefristete Aufenthalts- und Arbeitsgenehmigung Daten zur Ausbildung und Berufslaufbahn 13.6.1983: Abschluss des Studiums, Verleihung des Titels Diplomtierarzt 1983 - 984: Mitarbeit in einer Tierarztpraxis in Bad Hersfeld, Deutschland 1985 - 1987: Universitätsassistent an der Universität Ankara, Arbeit als wissenschaftlicher und klinischer Mitarbeiter am Institut für Geburtshilfe und Gynäkologie. Die Klinikarbeit erstreckte sich über alle Bereiche der Gynäkologie und Geburtshilfe. Eine Spezialisierung und Schwerpunktbildung erfolgte über die Puerperalphase des Rindes. Auch die an der Universität Ankara im Rahmen der fachtierärztlichen Ausbildung gehaltenen Vorträge bezogen sich auf dieses Schwerpunktthema (Anwendung des PGF2? bei der Endometritisbe-handlung im Puerperium des Rindes; Behandlungen der Ovarkrankheiten im Puerperium des Rindes). Zu den Aufgaben der Studentenbetreuung zählten das Abhalten von Übungen und Klinikvorträgen zu verschiedenen gynäkologischen Themen. 1987 - 1992: Doktoratsstudium, wissenschaftliche Forschung und klinische Arbeit an der Veterinärmedizinischen Universität Wien, Klinik für Geburtshilfe, Gynäkologie und Andrologie Hauptthemen der wissenschaftlichen Forschung waren: Puerperalkontrollen und deren Auswirkungen auf die Trächtigkeit des Rindes, Erkrankungen im Puerperium und Therapiemöglichkeiten beim Rind, Frühträchtigkeits- und Ovartätigkeitsuntersuchungen mittels Ultraschall und Anwendung von Hor-monen (GnRH, PGF2?, etc.) und Vitaminen (ß-Karotin, etc.) im Puerperium. Die Ergebnisse der Arbeiten wurden auf Vorträgen in Berlin, Gießen, Wels, München und Ankara vorgestellt. 25.6.1992: Promotion zum Dr. med. vet. an der Veterinärmedizinischen Universität Wien (Thema: Die Auswirkung verschiedener Behandlungen während der p.p.-Periode auf die Fruchtbarkeit von Kühen). 1992 - 1993: Wissenschaftliche Arbeiten, Doktorandenbetreuung und Unterrichtstätigkeit an der Universität Ankara, Institut für Geburtshilfe und Gynäkologie 22.10.1993: Erlangung des akademischen Titels Univ. Dozent. (Ankara) (Vorlesungen, Leitung der Klinikarbeit, Betreuung des Hochschulgutes, Bestandsbetreuung in verschiedenen Rinderbetrieben ) 1994 - 1997: Wissenschaftliche Arbeiten an der Veterinärmedizinischen Universität Wien, Klinik für Geburtshlife, Gynäkolgie und Andrologie Die Arbeiten umfassten weitere Untersuchungen der Frühträchtigkeit beim Rind (Gelbkörper- und Follikelmessungen, Feststellung von Hohlräumen des Gelbkörpers, Progesteron-, Vitamin E-, Folsäure-, B12-, Östrogenbestimmun-gen im Blut in verschiedenen Phasen der Frühträchtigkeit des Rindes). 1.3.1997 - Bestellung zum Gastprofessor an der Veterinärmedizinischen Universität 31.8.1997 Wien, Klinik für Geburtshilfe und Gynäkologie Lehrveranstaltung: "Frühembryonale Entwicklungsstörungen" 1999 Vortrag beim Symposium „Kleintierreproduktion“ anlässlich des 70. Geburts-tages von em. Prof. Dr. DDr. h. c. Kurt Arbeiter: „Abbruch der frühen Trächtigkeit bei der Hündin durch die kombinierte Anwendung eines Prostaglandins (Gabbrostim®) und eines Antiprolaktins (Galastop®).“ Symposium Kleintierreproduktion, 17.07.1999 Wien, Austria 17.10.2000 Ordentlicher Universitätsprofessor an der Universität Ankara 1989 - 2001 Wissenschaftliche Zusammenarbeit mit der Veterinärmedizinischen Universität Wien, zahlreiche Vorträge und Publikationen in Deutschland und Österreich (siehe Publikationsliste) 2000 DAAD-Stipendium für Deutschland (für 3 Monate) und Beenden der gemeinsam durchgeführten wissenschaftlichen Arbeit an der Tiermedizini-schen Fakultät der Ludwig Maximilians-Universität-München (Thema: Untersuchung der Veränderungen in der Durchblutung des Corpus luteum, der Follikel und der Lutealzysten mittels Farbdopplersonographie beim Rind). 2000 Homöopathie- und Phytotherapie in den Rinderbetrieben und Publikationen zu diesen Themenbereichen. Doktorandenseminare zu den Themen Homöopathie und Akupunktur 2001 Einladung der Tierärztekammer zu einemVortrag in Tirol Vergleichende Untersuchungen zur Behandlung der bovinen Endometritis mit intrauterinen Instillaten. Der Wiederkäuer im Alpenraum, Reproduktion und Neonatologie. Congress Centrum Alpbach. Tirol. Österreich., (2001) 2000 bis 2008 Durchführung zahlreicher Projekte gemeimsam mit A. Prof. Dr. Schäfer-Somi (Veterinärmedizinische Universität Wien) Schwerpunkte der Projekte waren folgende Themen: The Uterine and Placental Expression of Growth Factors, MMP 2,9 and MHC-I,II during Early Canine Pregnancy The Uterine and Placental Expression of MMP-2, -9, MHC-I,-II and Progesterone Receptors after Interruption of Early Canine Pregnancy with Aglepristone Immunological aspects during early canine pregnancy The expression of hormones, cytokines, T-cells, matrix metalloproteinases and major histocompatibility complex in maternal and foetal tissue, and the function of progesterone as a regulatory factor Expression of genes in the canine preimplantation uterus and embryo: implications for an active role of the embryo before and during invasion Expression of genes in the canine uterus and embryo Lymphocyte subsets in the pregnant canine uterus Durchführung zahlreicher Projekte mit Prof. Dr. Handler (Veterinärmedizini- sche Universität Wien; zur Zeit Professor an der LMU München) Schwerpunkte der Projekte waren folgende Themen: Frühgravidität und embryonale bzw. frühfetale Mortalität bei der Kuh – Gelb- körperdynamik, Progesteron-, Vitamin-E-, Vitamin-B12-, ?-Carotin-, und Folsäurekonzentrationen im peripheren Blut. Verbesserung der postpartalen Fertilität von Kühen durch Pulsatilla miniplex® Eignung der sonographischen Beurteilung von Ovardynamik und Uterusinvo- lution zur Fertilitätsprognose im Puerperium des Rindes. Wirksamkeit der intrauterinen Behandlung von puerperalen und post-puerpe- ralen Endometritiden mit Eucacomp® bzw. Lotagen® beim Rind 2000 - 2008 Beauftragung durch die Universitätskomission (YÖK) zur Bewertung der Publikationen und mündlichen Prüfung der Dozenturkandidaten als Mitglied der Prüfungskommission (Ankara) 2001 - 2008 Betreuung folgender Rinderbetriebe - Konya-Ereğli (Name des Betriebs; AKBEL): 1200 Kühe bzw. Kalbinnen, Kälber (Ausbildung der Tierärzte, Puerperalkontrolle, Östrussynchroni-sationen, Ultraschalluntersuchungen, prophylaktische Therapien, etc.). Im selben Betrieb getrennt gehaltene Schafe:1400 (Trächtigkeitsuntersuchun-gen, Östrussynchronisationen, Schwergeburt, Prolapsusus Uterie- bzw. Vaginae-Behandlungen, Behandlungen der metabolische Erkrankungen, Mastitistherapien und Haltungsmasnahmen, etc.) - Ankara-Elmadağ (Name des Betriebs; BESLER ENTEGRE): 110 Kühe bzw. Kalbinnen, Kälber - In der Umgebung von Ankara Untersuchungen in verschiedenen Rinderbetrieben (Betriebsgrößen zwischen 60 und 120 Kühe) und Behandlung von Schafen und Ziegen 2005 - 2008 Wissenschaftliche Zusammenarbeit mit Prof. Dr. B. Hoffmann (Veterinär- medizinische Fakultät, Klinik für Geburtshilfe, Gynäkologie und Andrologie, Justus-Liebig Universität Gießen) 2007 Zweiwöchiger wissenschaftlicher Aufenthalt an der Veterinärmedizinischen Universität Wien, Klinik für Geburtshilfe und Gynäkologie über das ERASMUS- Programm. Abhalten der Vorlesung „Endometritis beim Rind (Ursachen, Diagnostik, verschiedene Therapien und Prophylaxe) 2008 DAAD-Stipendium (Deutschland) für 2 Monate. Zusammenarbeit mit Prof. Dr. H. Bollwein (Thema: Untersuchungen über die Auswirkung von GnRH und hCG auf die follikuläre und luteale Durchblutung des Rindes). 2008-2011 Lehre, Dissertationen, Vorträge, Seminare, Forschung an der Veterinärmedizinische Fakültät Ankara. 2011-…… Senior Scientist (Projekte mit der Veterinärmedizinische Universität Wien; Klinik für Geburtshilfe und Gynäkologie Centre for Artificial Insemination and Embryo Transfer; Wissenschaftliche Arbeit mit A.Prof.Dr. Sabine Shäfer- Somi) Wietere Projekte mit den Fakültäten Zurich (Bollwein, Kowalewski), Berlin (Prof.Dr. Johennes Handler), Ankara, Samsun, Aydın und Kars, Burdur Mitgliedschaften und Aufgabenbereiche: ESDAR (The european society for domestic animals reproduction) Türkischer Verein für Buiatrik Türkische Gesellschaft für Geburtshilfe und Gynäkologie Kommisionsmitglied der Habilitationsprüfung Kommissionsmitglied für die Beurteilung der im Ausland studierenden Assistenten Leiter für wissenschaftliche Angelegenheiten President der Homöopathie-Verein Ankara Kooperationen: Wien Prof. DDR. h.c. Kurt Arbeiter (Klinik für Geburtshilfe, Gynäkologie und Andrologie) – 1987 – 1998 Prof. Dr. Jörg Aurich, A.Prof. Dr. Sabine-Schäfer-Somi, Prof. Dr. Johannes Handler (Klinik für Geburtshilfe, Gynäkologie und Andrologie) – 1998 bis heute A.Prof. Dr. I. Walter (Institut für Histologie und Embryologie) – 2000 bis heute Ass. Prof. Dr. M. Platzl (Institut für Immunologie) – 2006 bis heute München: Prof. Dr. Rudolf Stolla (Tiermedizinische Fakultät der Ludwig Maximilians- Universität-München) – 2000 - 2002 Hannover: Prof. Dr. Heinrich Bollwein (Klinik für Rinder, Stiftung Tierärztliche Hoch- schule Hannover) – 2000 bis heute Gießen: Prof. DDr. Bernd Hoffmann (Klinik für Geburtshilfe, Gynäkologie und Andro- logie, Justus-Liebig Universität Gießen) – 2005 bis heute Leipzig: Prof. Dr. A. Sobiraj (Tierärztliche Fakultät der Universität Leipzig) – ab 2008 Schweiz: Dr. Kowalewski (Veterinärmedizinische Fakultät der Universität Zürich); 2009-……….. Prof.Dr. Bollwein (Veterinärmedizinische Fakultät der Universität Zürich); 2012-…………….. Interessen: Computer (Vista, SPSS, Power Point, Exel Adobe Photoshop 7.0, u.a.m.) Statistische Auswertungsmodelle klassische Musik, Theater und Kino, Literatur Homöopathie, Akupunktur Türkçe: İlk, Orta ve Lise Öğrenimini Ankara’da yaptı. Daha sonra Almanya’da (Bremen) Almanca öğrendi ve Bremen’de başladığı işletme iktisat eğitimini bırakarak Veteriner Hekimlik eğitimini seçti. Uludağ Üniversitesi Veteriner Fakültesini 1983 yılında bitirdi. Frankfurt’a yakın Bad-Hersfeld kasabasında bir Veteriner Hekim Aile’nin yanında Asistan Veteriner Hekim olarak çalıştı. 1985 yılında Ankara Üniversitesi Veteriner Fakültesine Araştırma Görevlisi olarak başladı. 1987 yılında kendi yazığı bir projenin kabul edilmesi sonucu Avusturya Hükümetin’den aldığı bursla (ÖAAD) başta proje çalışması ve arkasından doktora ve klinik alanında (büyük, küçük hayvan) Avustuya’da Prof.DDr.K. Arbeiter ile birlikte çalıştı. Bu süreç içerisinde Münih, Giessen,Ulm ve Berlin’de Fertilite ve Sterilite alanında kongrelerde bildiri sundu. 1992 ve 2011 tarihlerinde her biri iki ay olmak üzere değişik çalışma ve yöntemleri görmek üzere Amerika’da bulundu. 1992 yılında doçent ve 2000 yılında Profesör oldu. Bu süreç içerisinde birçok kez birlikte çalışmak üzere Viyana Veteriner Fakültesine davet edildi ve bu davetler sonucunda Türkiye ve Avusturya arasında bilimsel işbiliği olanakları çerçevesinde birçok çalışma yapıldı. Almanya’nın birçok kentinde (Berlin, Münih, Giessen, Hannover, Leipzig) ve İsviçre, Avusturya, Slovenya, Belçika, Polonya, Macaristan, Almanya, Hollanda, İtalya…. gibi ülkelerde kendi ve asistanları ile birlikte birçok kongreye katıldı, ileri jinekolojik teknikler üzerine sunular yaptı. 1998 yılında Viyana Üniversitesinde misafir profesör olarak görev aldı. 1987 yılından başlayarak Viyana Veteriner Üniversitesi Araştırma ve Geliştirme Çiftliğinde Prof.DDr. K. Arbeiter’ın eşliğinde hem öğrenci eğitimi, araştırmalar ve sürüyönetim, idare gelişim ve sağlığı alanında çalıştı, daha o dönemden başlayarak ultrasonografik tanıyı gebelik, siklus tayini ve embryonik ölümlerde uyguladı. Daha sonra ki yıllarda renkli doppler tanı yöntemlerini Prf.Dr. Bollwein (halen Zürich Üniversitesi Veteriner Fakültesi, Sığır Kliniği Bölüm Başkanı'dır) ile birlikte pratiğe uyguladı. DAAD Bursu ile Münih ve Hannover’de renkli doppler ile corpus luteum kan akışkanlığı üzerinde çalışmalar yaptı. Halen Viyana Veteriner Üniversitesi Doğum , Jinekoloji, Androloji ve Suni Tohumlama , Zürich Üniversitesi Anatomi-Histoloji , Danimarka Doğum Kliniği ve ayrıca Sığır Kliniği ile ve Türkiye’de ki değişik Fakültelerle birlikte bilimsel çalışmalarını uluslararası projeler bazında ve Workshoplar tarzında sürdürmektedir . Ayrıca değişik ülkelerde ve Türkiye’de “Reprodüktif Sürü İdaresi” alanında genel sürü sağlığının sağlanması, fertilite parametrelerinin iyileştirilmesi, etki eden etmenlerin belirlenmesi ve önlemler alınarak sağlıklı sürü yetiştirilmesi alanında 1989 yılından bugüne kadar çalışmaktadır. Avusturya Veteriner Hekimler Odasının daveti ile “İneklerde Endometritis Tedavi Seçenekleri ve Yenilikler”, Prof.Dr. K. Arbeiter için düzenlenen kongrede “Köpeklerde Abort amacıyla Cabergolin kullanım ve Uygulmaları”, İsviçre’de davet üzerine “Kedi ve Köpekte güncel kontrasepsiyon yöntemleri” ve değişik davetlerde değişik ülkelerde bu konular üzerine konferanslar vermiştir. Almanya’da Prof.Hoffmann (Giessen), Prof.Dr.Leidl ve Stolla (Münih), Avusturya’da (Prof.Dr. K.Arbeiter, Prof.Dr. Schaefer-Somi, M.Drillich), İsviçre’de (Prof.Dr. H.Bollwein, Prof.Dr. Boos ve A.O. Prof.Dr. Kowalewski), Danimarka Kopenhagen (AO Prof.Dr. Sandra K. Georicke-Pesch ile çalıştı ve halen çalışmaktadır. Değişik ulusal ve impact factor bakımından Veteriner Doğum ve Jinekoloji Alanında en yüksek olan uluslararası dergilerde (Reproduction, Animal Reproduction Science, Theriogenology, Reprod Dom Anim) yayınları çıkmış ve halen yurtdışındaki ve Türkiye’de ki çalışma grubu ile birlikte yayın yapmaktadır. Ve bu dergilerin hepsinden “reviewer” olarak gelen yayınların değerlendirmesi için kendisine yayın gönderilmektedir. Birçoğu uluslararası olmak üzere 86 yayını vardır ve halen süren projelerden yeni yayınlar hazırlanmaktadır. Değişik ülkelerde katıldığı veya sunu yaptığı kongre sayısı elli’nin üzerindedir. Çalışmalar genellikle uygulamayı açıklayıcı reprodüktif immünoloji, reprodüktif moleküler biyoloji veya anne yavru interaksiyonu ve uterus yangıları, abortus…… üzerine yapılmakta ve sürekli bu alanda değişik ülkelerde bilgi alış verişi olmaktadır. Sığırlarda ise “Corpus luteum kan akışkanlığı, endometritislerde yeni tanı yöntemleri ve hormonların uygulamada kullanımı, ekotekstür, Akupunktur ve Corpus luteum kan akışkanlığı, puerperium ” gibi konular üzerine yapılmaktadır. Köpeklerde kontrasepsiyon (çiftleşmenin engellenmesi, erken dönemde istenmeyen gebeliklerin medikal yöntemlerle bitirilmesi), doğum sonrası dönem, gebelik dönem patolojileri ve meme tümörü gibi birçok alanda uluslar arası düzeyde yayınlanmış eserleri bulunmaktadır. Yayınlarına “PubMed).” bilimsel iletişim sitesinden ulaşmak mümkündür. Özel çalışma alanlarından biride alternatif tedavi metodlarıdır (Homöopathie ve Akupunktur) ve gerektiğinde tevdide bu yöntemlerini de kullanmaktadır. Alternatif tedavi teknikleri eğitimini Viyana Veteriner Üniversitesinde Prof.Dr.Kothbauer’den almıştır. Prof.Dr. Selim Aslan evli ve bir çocuk babasıdır. ESDAR (European Society for Domestic Animal Reproduction) üyesidir.
Near East University, Obstetrics and Gynaecology; veterinary Faculty
Veterinaer Universitaet Wien, Vetsuisse-Faculty University of Zurich, Viyana (Doğum) , Zürich (Anatomi-Histoloji, Moleküler Biyoloji, Sığır Kliniği)Kopenhagen (Büyük hayvan Kliniği)
Veterinaermedizinische Universitaet Wien, Geburtshilfe, Gynaekologie und Andrologie
Stiftung Tieraerztliche Fakultaet Hannover, Klinik für Geburtshilfe u. Gynaecology
Ankara Üniversitesi, Doğum ve Jinekoloji
Ludwig Maximillan Universitaet-München, Doğum ve Jinekoloji
Ankara Üniversitesi, Doğum ve Jinekoloji Ana Bilim Dalı
Veterinaermedizinizche Universitaet Wien, Geburtshilfe, Gynaekologie, Andrologie
Dr. vet. med. Heinze, Klinik, İneklerde Sürü idaresi ve Sağlığı (Herd-Management)
Doğum , Jinekoloji, Obtetrik, Androloji ve Suni Tohumlama
Veterinaer Universitaet Wien
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Veteriner Fakültesi
Background: The focus of our recent studies (Kowalewski et al., 2009, 2010, 2011a,b) was directed towards the cellular- and molecular mechanisms leading to the onset of parturition in the dog. In order to gain some more information about the molecular and endocrine events governing this process experiments were performed using the competitive progesterone receptor blocker - Alizine (Virbac). We observed similar changes in the utero/placental prostaglandins system during normal and Alizine-induced parturition/abortion. Our main findings pointed towards the progesterone-receptor mediated alterations in the feto-maternal communication, between the maternal decidual cells (the only cells in the canine placenta expressing progesterone receptor) and the fetal trophoblast cells, as the main trigger for the prepartal PGF2? output in the dog. But „similar“ does not mean „identical“. From the clinical point of view it is known that the uterine contractions after the application of Alizine seem to be less pronounced than during the normal parturition. This becomes more obvious when Alizine is being applied in the mid- and late- pregnancy, where fetal resorption is not anymore possible and the application of Alizine must result in an abortion. The action of Alizine beyond 45th day of pregnancy is diminished. From the clinical point of view-administration of this compound as monothepeutic at 3rd timester of pregnancy may not induce the foetal expulsion. Therefore, clinicians have developed several protocols comprising e.g. the usage of oxytocin or PGF2? in order to improve the process of parturition. The effects of Alizine on the cessation of the luteal steroidogenic function (= decrease of peripheral progesterone levels) and uterine contractions and finally on the outcome of parturition seem to depend upon the timepoint of Alizine application during the course of pregnancy Goal: The main goal of the ongoing project is: - to investigate the effects of the different timepoints of Alizine application (early-, mid-, late- pregnancy) on the utero/placental prostaglandin-synthesis, luteal steroidogenesis and luteal as well as uteroplacental hormonal/receptor status. Significance of the planned work: With this project we expect to provide clinically active colleagues with new information that could be helpful in optimizing and developing of new treatment protocols. From the scientific (basic sciences) point of view we expect a significant contribution to the understanding of endocrine mechanisms regulating canine reproduction.
(1) University of Copenhagen (UCPH), Department of Large Animal Science, Faculty of Health and Science, Copenhagen University, Denmark, with registered office at Copenhagen, registered under number cvr. 29 97 98 12, represented by Mr Hans Henrik Dietz, Department Head (the “Principal Investigator”). (2) University of Ondokuz Mayıs (UOM), Faculty of Veterinary Medicine, Department of Clinical Sciences, 55200, Samsun, Turkey, represented by Prof. Murat Findik (the “Sub-Contractor”). (3) Prof. Selim Aslan (SA), Nera East University, Veterinary Faculty, Obstetrics and Gynecology, Nicosia-TRNC, CYPRUS, will serve as a coordinator of the study – responsible for coordination of the study at and with UOM and communication with Assoc. Prof. Dr. Sandra Goericke-Pesch from UCPH (the “coordinator”) Project description: The downregulated dog and recrudescence of spermatogenesis The aim of the study is to characterise downregulation of germinative and endocrine testicular function following treatment with a 4.7 mg deslorelin implant in dogs and recrudescence after abolition of downregulation by implant removal. Furthermore, full reversibility of all treatment-induced effects should be confirmed by hemi-castration of 4 dogs at the time of downregulation and weekly follow-ups until return to pre-treatment semen parameters followed by removal of the second testis. Experimental design: All dogs will be submitted to a clinical and andrological examination including measurement of testicular size (length, width, height) and assessment of semen quality. Only healthy dogs without andrological deviations and semen parameters within the normal range as defined by Günzel-Apel et al. (1994) Riesenbeck et al. (2001) and Pesch et al. (2007) (see below) will be included. Day of examination and inclusion into study will be defined as day 0. A blood sample will be collected for hormone measurements (testosterone (T), estradiol-17ß (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH) concentrations etc.). The dogs will be randomly assigned to the following two treatment groups: Group 1 (n=5): control; castration after clinical examination and preservation of testicular and epididymal tissue for further examinations according to the protocol below. Group 2 (n=45): treatment with a slow release GnRH-agonist implant (Suprelorin®, 4.7 mg deslorelin) subcutaneously at the paraumbilical region on day 0, blood sampling and andrological examination including measurement of testicular size on days 28, 56 and 84 (= weeks +4, +8, +12), implant removal under local anaesthesia after 5 months (week 0), surgical castration of four to five dogs each at weeks 0, 1, 2, 3, 4, 5, 6, 8, 10. Following implant removal, weekly blood sampling and andrological examination until castration. On the day of castration, blood sampling, testicular measurements before castration and preservation of testicular and epididymal tissue for further examinations (figure 1). Except for four dogs in week 0, both testes will be removed at the time of castrations. In week 0, hemicastration will be performed in four dogs (figure 2). Weekly semen collections until return to normal (before-treatment) semen parameters (week x) should be performed followed by surgical removal of the second testis (week x+1) and preservation of testicular and epididymal tissue for further examinations.
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Yayınlar 2017 Morphological, histological and molecular investigations on canine uterine tissue after ovariectomy S Schäfer-Somi, K Deichsel, H Beceriklisoy, D Korkmaz, I Walter, S Aslan Theriogenology 102, 80-86 2017 Functional implications of the utero-placental relaxin (RLN) system in the dog throughout pregnancy and at term M Nowak, A Gram, A Boos, S Aslan, SS Ay, F Önyay, MP Kowalewski Reproduction 154 (4), 415-431 2017 Expression of GnRH receptor in the canine corpus luteum, and luteal function following deslorelin acetate-induced puberty delay D Kaya, A Gram, MP Kowalewski, S Schäfer-Somi, M Kuru, A Boos, ... Reproduction in Domestic Animals 2017 Early pregnancy diagnosis in dairy cows by ultrasound and determination of bovine-pregnancy-associated glycoproteins, bovine pregnancy specific protein B and progesterone O Ergene, I Darbaz, S Sayiner, S Aslan REPRODUCTION IN DOMESTIC ANIMALS 52, 83-83 2017 Uterine responses to early pre-attachment embryos in the domestic dog and comparisons with other domestic animal species FR Graubner, A Gram, E Kautz, S Bauersachs, S Aslan, AR Agaoglu, ... Biology of Reproduction 97 (2), 197-216 2017 Kisspeptin-10 and the G protein-coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells S Schäfer-Somi, SS Ay, D Kaya, M Sözmen, HB Beceriklisoy, AR Ağaoğlu, ... Reproduction in Domestic Animals 52 (S2), 123-129 1 2017 Effect of deslorelin on testicular function, serum dihydrotestosterone and oestradiol concentrations during and after suppression of sexual activity in tom cats N Gültiken, S Aslan, SS Ay, MY Gülbahar, J Thuróczy, E Koldaş, D Kaya, ... Journal of feline medicine and surgery 19 (2), 123-131 1 2017 The Assessment of Diagnostic and Ultrasonographic Findings in a Bitch with True Vaginal Prolapse I Darbaz, O Ergene, E Canooglu, C Gultekin, S Aslan Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23 (5), 843-846 2017 Koyun ve Keçilerde Metabolik Problemler ve Reprodüksiyona Etkileri S ASLAN, İ DARBAZ, O ERGENE Turkiye Klinikleri Journal of Veterinary Sciences-Obstetrics and Gynecology … 2017 Ovarian Tumour in a Bitch: Diagnosis, Surgery and Recovery I Darbaz, O Ergene, G Sonmez, S Aslan Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23 (5), 839-842 2017 Pregnancy Markers in Serum of Bitches S Schäfer-SOMI, S ASLAN Turkiye Klinikleri Journal of Veterinary Sciences-Obstetrics and Gynecology … 2017 Association of luteal blood flow with follicular size, serum estrogen and progesterone concentrations, and the inducibility of luteolysis by PGF 2? in dairy cows S Kaya, C Kaçar, B Polat, A Çolak, D Kaya, İS Gürcan, H Bollwein, ... Theriogenology 87, 167-172 2 2017
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Transmissible venereal tumour (TVT) is a rare vaginal tumour that can be treated surgically or cryosurgically as well as by radiotherapy or chemotherapy. Vincristine has been found to be very effective for treating TVT. Since vaginal secretion or discharge may contain neoplastic cells, the cytological identification of TVT cells is possible. The present study was carried out in 12 bitches. Vaginal smears were obtained with cotton swab from the anterior vagina and TVT suspected structures. The smears were stained according to Papanicolaou and assessed by light microscopy. Additionally the general condition of the patients was evaluated by haematological and radiographic examinations. In bitches with TVT vincristine sulphate was administered intravenously at weekly intervals. The total treatment period was three to six weeks until no atypical cells were found in the smear. This was the case after an average of 3.2 +/- 1.3 applications. Tumour masses became smaller and by this non-visible from the rima vulva after 4.2 +/- 0.7 applications. During the treatment, two of the 12 bitches (16.7%) suffered from vomiting and diarrhoea while three (25%) showed neutropenia. Twelve months after completion of treatment, the bitches were examined again and vaginal smears were taken in order to control the recovery process or a possible recurrence of TVT. No atypical cells were found in any vaginal smear. By this exfoliative cytology has proved to be a safe and easy method for TVT diagnosis and for observing the recovery process.
english
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The efficacy of cabergoline solely or combined with a PGF2alpha analogue in inducing abortion at different stages of pregnancy was investigated in 18 queens. The queens were assigned to two treatments: Group I ( n=10 )-cabergoline (15 microg/kg; daily, orally) and Group II ( n=8 )-cabergoline (15 microg/kg; daily, orally) combined with alfaprostol (10 microg/kg; every other day, subcutaneously). Each group was divided into two subgroups according to the duration of pregnancy when treatments started: Group IA ( n=8 ) included queens from Days 34 to 42 after mating. Group IB cats ( n=2 ) started treatments on Day 45 post-mating. Similarly, the combination of cabergoline and PGF2alpha analogue was first given to Group IIA ( n=6 ) from Days 25 to 40 of pregnancy and to Group IIB ( n=2 ) on Days 45 and 47, respectively. Termination of pregnancies was successful in all cats of Group IA, while treatments failed in both cats of Group IB, even though seven and eight treatments, respectively, were given. Mean (+/-S.D.) plasma progesterone concentrations before the start of treatments were 85.0+/-12.3 nmol/l and decreased within 3 days to 8 nmol/l and subsequently to basal values, when the queens aborted (Group IIA, n=6 ) or gave birth prematurely (Group IIB, n=2 ). When abortions failed (Group IB, n=2 ), progesterone concentrations remained elevated (16.9 and 9.8 nmol/l). Duration of combined therapy during late pregnancy in Group IIB ( n=2 ) lasted about 10 days. In both cases, premature birth occurred and the kittens died within 16 h after birth. Overall, treatments starting on Days 25-42 of pregnancy (Groups IA and IIA) had abortion rates of 100%. The average duration of treatments was 5.6+/-1.5 days (range, 3-8). Side effects seen were vomiting and occurred in 6 of the 109 (5.5%) treatments. In conclusion, pregnancies were successfully terminated in the second trimester of feline pregnancy by daily application of cabergoline solely or combined with the PGF2alpha analogue alfaprostol (given every other day). Cabergoline alone was ineffective in inducing abortion at later stages of pregnancy.
Englisch
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The aim of the present study was to demonstrate the presence and localization of MMP-2 and -9 by means of RT-PCR and immunohistochemistry (IHC) within the canine uterus from the pre-implantation stage until mid-gestation and to determine MMP-2 and -9 activities by means of zymography. For this purpose, samples of the uterus and salpinx from bitches were obtained after ovariohysterectomy. Pre-implantation stages (5–12 days after mating, n = 11) were determined by verifying embryos after flushing the uterus. Further groups were determined as implantation (15–19 days after mating, n = 9), post-implantation (20–30 days after mating, n = 9) and placental stages (30–45 days after mating, n = 3). A non-pregnant group (17–30 days after mating, n = 4) served as control. MMP-2 and -9 positive cells were detected in all specimens from pregnant and nonpregnant bitches, however, with different distributions. MMP-2 was present in endothelium and smooth muscles of blood vessels and the myometrium of pregnant and nonpregnant bitches, additionally in the surface epithelium of the oviduct. The latter also stained positive for MMP-9. During placentation, MMP-2 was detected mainly in fetal blood vessels and trophoblastic cells. Higher MMP-2 activity was observed in the endometrium and myometrium of all pregnant groups compared with the nonpregnant group (p < 0.05). The pregnant groups did not differ significantly from each other (p > 0.05). MMP-9 was present in blood vessels, smooth muscle cells and epithelia, such as maternal surface epithelial cells, uterine crypts and glands. During placentation, the deep uterine glands and the epithelium of the glandular chambers were immunoreactive to MMP-9. Highest MMP-9 activities were reached in the endometrium of the pre-implantation group (23.2% of total MMP-9) and placental parts (33.3%).
English
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The aim of the present study was to investigate the effects of two medications on two subsequent abortions and plasma hormone concentrations of dogs. For this purpose, two groups of bitches (n = 5 each), received the antiprogesterone aglepristone (Alizine®) at 10 mg/kg body weight on two subsequent days around day 30 after mating. In group II, the antiprolactin cabergoline (Galastop®) was additionally administered po at 5 µg/kg body weight until the start of abortion. The plasma concentrations of relaxin, progesterone (P4) and estradiol-17ß (E2) were measured before, during and after each abortion. During the next cycle after the abortion, the same bitches were mated again and in pregnant animals, induction of abortion was performed as before. During the third cycle, pregnant bitches were allowed to whelp. Termination of first pregnancy occurred significantly earlier after the combined treatment (6.8 versus 10.6 days, p < 0.05). In both groups and during both abortions, relaxin varied between individuals; however, there was a continuous decrease after the abortions and no significant differences between groups (p > 0.05). In one bitch with high relaxin concentrations before treatment (11.6 ng/ml), a cystic endometrial hyperplasia was diagnosed. In the aglepristone only group, P4 concentrations increased significantly after the first application (p < 0.05), then decreased continuously until day 45 after the beginning of abortion. In the combined group, there was a continuous decrease until day 45 (p > 0.05). At this time, P4 concentrations between 0.47 and 84.9 nmol/l were measured in both groups. The level of E2 over time was not influenced by any medication. We therefore note that the two medications mainly influenced plasma concentrations of P4 in different ways, probably due to specific treatment-hormone interactions. However, all measurements fell within the range considered normal.
English
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Uterine tissue from pregnant bitches was investigated by qualitative RT-PCR for the gene expression of local factors potentially important for the implantation of canine embryos. For this purpose, 10 bitches identified as being at the time of implantation or early placentation by means of ultrasonography before ovariohysterectomy (days 20-35, n = 10) provided tissues for comparison to tissue collected in a previous study and identified as early pregnant (n = 10) or non-pregnant (n = 4) by embryo flushing after ovariohysterectomy (days 10-12 after mating; Schäfer-Somi et al. 2008). Uterine tissue was excised from the middle of the left horn from early pregnant and non-pregnant animals, including from interplacental and placentation sites. The following genes were investigated: CD-4, -8; cyclooxygenase (COX)-1, -2; granulocyte macrophage-colony stimulating factor (GM-CSF); hepatocyte growth factor (HGF); insulin-like growth factor (IGF)-1, -2; transforming growth factor (TGF) and tumour necrosis factor (TNF)-alpha; interferon (IFN)-gamma; interleukin (IL)-1beta, -2, -4, -6, -8, -10, -12; leukaemia inhibitory factor (LIF) and leptin. Gene expression for CD-8, COX-1, TGF-beta, HGF, IGF-1, IL-2, -4,-10, IFN-gamma and LIF were detected in the pre-implantation uterus, and all except IL-2 and -10 were still detectable during the implantation and placentation stage. During implantation, mRNA for IGF-2 and GM-CSF were additionally detected. The dioestrous uterus differed from the pregnant uterus because of the absence of CD-8, IL-4 and IFN-gamma and the expression of CD-4, TNF-alpha and IL-6. The results suggest that IL-4, IFN-gamma, CD-8, GM-CSF and IGF-2 are regulated in a pregnancy-specific manner and that GM-CSF and IGF-2 probably have growth supporting and immune modulating functions during implantation of the canine embryo.
english
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This study was designed to assess endocrine changes associated with termination of mid-term pregnancy after use of two different protocols. For this purpose we compared the effects of aglepristone (AGL) alone and in combination with cloprostenol (CLO) on serum concentrations of progesterone (P4), estradiol (E2) and relaxin (RLN) measured at short-term intervals during the abortion period in bitches. Fourteen pregnant bitches between day 25 and 32 of gestation were used in the study. In the AGL group (n = 7), aglepristone was administered solely (10 mg/kg body weight (BW), subcutaneously, once daily on two consecutive days) whereas in the AGL-CLO group (n = 7), aglepristone (dosage as in AGL group) and cloprostenol (1 µg/kg BW, subcutaneously, same with aglepristone) were combined. All pregnancies were successfully terminated 5.2 ± 1.6 days after initiation of treatments, which was significant in both groups (P > 0.05). At the time of the start of abortion (SA) and the end of abortion (EA), the mean P4 concentrations were 26.6 ± 7.3 and 12.0 ± 6.4 ng/ml in AGL group, and 2.7 ± 0.7 and 0.9 ± 0.1 ng/ml, in AGL-CLO group, respectively (P < 0.01). Serum E2 concentrations were significantly higher (P < 0.05) in AGL group at 42, 48, 54 h and SA after initiation of treatment. In the AGL-CLO group, serum RLN concentrations did not significantly change from the initiation of treatment to EA (P > 0.05). However, markedly higher RLN concentrations (P < 0.05) were observed in the AGL group at 48 h (1.5 ± 0.7 ng/ml) and at SA (1.6 ± 0.5 ng/ml). The results of the present study indicate that changes in the hormonal concentrations affect the mechanism of abortion in different ways. Further in depth studies investigating changes in the expression of hormone receptors inside the ovary, endometrium and placenta might be helpful to our understanding of the endocrinological differences observed in this study.
Yayın ingilizcedir.
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The aim of the present study was to compare the clinical and endocrinological effects of different applications of misoprostol (MIS) and aglepristone (AGL) for the induction of abortion in bitches. For this purpose, 28 healthy pregnant bitches from different breeds, ages, body weights (Body weigt, BWs, 10–40 kg), and between Days 25 to 35 of gestation were used. Bitches were randomly assigned to four groups. In group 1 (GI, n = 7), AGL (10 mg/kg BW, s.c. on 2 consecutive days); in group 2 (GII, n = 7), AGL (as in GI), intravaginal MIS (IVag, 200 µg for bitches with ?20 kg BW, 400 µg for bitches with >20 kg BW, daily intravaginally until completion of abortion); in group 3 (GIII, n = 7), AGL (as in GI), ICVag (as in GII), per os MIS (400 µg for bitches with ?20 kg BW, 800 µg for bitches with >20 kg BW, daily orally, until completion of abortion); in group 4 (GIV, n = 7), AGL (as in GI), per os MIS (as GIII) were used. Clinical, vaginal, and ultrasonographic examinations were performed daily until abortion was completed. For measurement of serum progesterone, blood samples were collected in all groups immediately after the first AGL administration and every other day until completion of abortion. No statistical differences were found between groups concerning the duration until completion of abortion after treatment (nonsignificant); however, in GII, one bitch completed abortion 2 days after the start of treatment.
Yayın ingilizcedir.
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Although there is no acute luteolytic mechanism in the absence of pregnancy in the bitch, a precise and well-timed embryo–maternal interaction seems to be required for the initiation and maintenance of gestation. As only limited information is available about these processes in dogs, in this study, the uterine expression of possible decidualization markers was investigated during the pre-implantation stage (days 10–12) of pregnancy and in the corresponding nonpregnant controls. In addition, the expression of selected genes associated with blastocyst development and/or implantation was investigated in embryos flushed from the uteri of bitches used for this study (unhatched and hatched blastocysts). There was an upregulated expression of prolactin receptor (PRLR) and IGF2 observed pre-implantation. The expression of PRL and of IGF1 was unaffected, and neither was the expression of progesterone- or estrogen receptor b(ESR2). In contrast, (ESR1) levels were elevated during early pregnancy. Prostaglandin (PG)-system revealed upregulated expression of PGE2-synthase and its receptors, PTGER2 and PTGER4 and of the PG-transporter. Elevated levels of AKR1C3 mRNA, but not the protein itself, were noted. Expression of prostaglandin-endoperoxide synthase 2 (PTGS2) remained unaffected. Most of the transcripts were predominantly localized to the uterine epithelial cells, myometrium and, to a lesser extent, to the uterine stroma. PGES (PTGES) mRNA was abundantly expressed in both groups of embryos and appeared higher in the hatched ones. The expression level of IGF2 mRNA appeared higher than that of IGF1 mRNA in hatched embryos. In unhatched embryos IGF1, IGF2, and PTGS2mRNA levels were below the detection limit.
Yayın ingilizcedir
Objective: The aim of this study was to evaluate changes in the endome- trium by using echotexture parameters during and after treatment of en- dometritis with intrauterine administration of an intra ute rine antiseptic solution (Lotagen®, 3% metacresolsulphonic acid and formaldehyde) in cows which became pregnant after treatment. Material and methods: According to the severity of endometritis 21 cows were divided into three groups: E1 (slight, n = 7), E2 (moderate, n = 8), E3 (severe, n = 6). The control group (C, n = 11) consisted of cows without endometritis that did not receive an intrauterine medication. A software (Bs200 Pro®) was used to evaluate echotexture parameters Contrast (CON), Gradient (GR), Homogeneity (HOM), Mean Gray Level (MGL) of images taken during the examinations at hours (h) 0, 1 and 6 and days (d) 2, 3, 5 and 10. Results: At 0 h, GR was significantly lower in group E2 than in groups E1 and C (p < 0.05). There was an increase in GR values between 0 h and 10 d in group E2 and E3, but a decrease during the same time interval in group C (p < 0.05). In contrast, CON values of group E2 were lower (p < 0.05) at 0 h compared to other timepoints of examination and lower than in group C. HOM values were lower (p < 0.05) in groups E1, E2 and E3 than in group C on d 5 and d 10. HOM values were higher at 1 h compared to 6 h, d 2 and d 10 in group E3 (p < 0.05). By contrast to GR values, HOM values were higher in group C at 6 h and d 10 than they were in group E3. MGL values of group E2 were higher (p < 0.05) than in group C until d 10 and higher (p < 0.05) in group E3 than in group C at 6 h after treat- ment. In group E2 an increase of MGL values until d 2 was followed by a decrease (p < 0.05). Conclusion: Echotexture parameters determined by the evaluation of sonographic B-mode images reflect changes in the en- dometrium and could be used for the evaluation of the recovery period after treatment of endometritis.
Yayın ingilizcedir.
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In this study, effect of acupuncture (AP) on the luteal size (LS), luteal blood flow (LBF) and progesterone (P4) levels were investigated in the presence of corpus luteum (CL) in cows. Seven days after 14-days interval PGF2? estrus synchronization protocol, CL positive animals were assigned either to a control group (AP–, n=10) or to an AP group (AP+, n=10) stimulated by using B22 and B23 sensitive acupoints. LS and LBF examinations were carried out before the stimulation (0h) and at 1st, 3rd, 6th hour on d7 and thereafter on d9, d10, d11, d12 and d13 following the AP stimulation in each group with a portable color Doppler ultrasonography. Blood samples for P 4 measurement were collected during each examination. There was no significant difference in LS, LBF, or P 4 mean values between groups. However, LBF significantly increased at 6h after stimulation (P<0.05) in AP+ group but it increased at d11 in AP– group (P<0.05). The significant increase in LS was observed earlier in AP+ group (on d9; P<0.01) than AP– (on d11; P<0.05). Serum P 4 concentrations increased at 3h, d9 and d10 in AP+ group (P<0.05), however a significant difference was only observed at 3h in AP– group (P<0.05). In conclusion, AP stimulation induces earlier increases in LS, LBF and P4 parameters in cows during luteal phase
Bu çalışmada akupunkturun (AP) ineklerde korpus luteum (CL) varlığında luteal büyüklük (LS), luteal kan akımı (LBF) ve progesteron (P4) seviyeleri üzerindeki etkisi incelendi. 14 gün aralıklı PGF2? östrus senkronizasyon protokolünden 7 gün sonra, CL tespit edilen hayvanlar kontrol (AP–, n=10) ve duyarlı B22 ve B23 kullanılarak stimüle edilen akupunktur gruplarına ayrıldı (AP+, n=10). LS ve LBF incelemeleri taşınabilir bir renkli Doppler ultrasonografi ile 7. günde stimülasyon öncesi (0. saat), izleyen 1, 3, 6. saatler ve 9, 10, 11, 12 ve 13. günlerde yapıldı. Her muayenede P 4 ölçümleri için kan örnekleri alındı. Gruplar arasında LS, LBF ve P 4 ortalama değerleri farklı değildi. Bununla birlikte, LBF AP+ grubunda stimülasyon sonrası 6. saatte (P<0.05) ancak AP– grubunda 11. günde arttı (P<0.05). LS’deki önemli artış ise AP+ grubunda (9. gün; P<0.01), AP– grubuna göre (11. gün; P<0.05) daha erken gözlendi. Serum P 4 konsantrasyonları AP+ grubunda 3. saat, 9. gün ve 10. günde artış gösterdi (P<0.05). Ancak AP– grubunda sadece 3. saatte önemli değişim gözlendi (P<0.05). Sonuç olarak, luteal faz sırasında akupunktur stimülasyonu LS, LBF ve P4 değerlerinde daha erken artışa neden olmakta
The objective of this study was to evaluate pregnancy rates to timed artificial insemination (TAI) following presynchronization with a single dose of PGF2? ten days before CoSynch-56 protocol in Brown Swiss crossbred cows and heifers. Group I (GI) cows (n=89) and heifers (n=39) received PGF2? ten days prior to Cosynch-56 protocol while Group II (GII) cows (n=82) and heifers (n=38) received CoSynch-56 protocol only. Pregnancy was evaluated using ultrasonography 30-35 days after TAI. Pregnancy rates did not differ significantly between GI and GII (34.4% and 28.3 %, respectively, P>0.05). However, pregnancy rates differed significantly (P<0.05) between cows and heifers in GII (34.1% and 15.7%, respectively). Furthermore, pregnancy rates of heifers in GI was significantly higher than that of heifer in GII (P<0.01, 41.0% and 15.7%, respectively). Notably, pregnancy was not achieved in GII heifers with plasma P4 values < 1ng/ml. In conclusion, presynchronization with PGF2? before Cosynch-56 protocol did not enhance pregnancy rates in Brown Swiss cows but did in heifers. Also, high P4 levels may enhance overall pregnancy rates of cows and heifers subjected to the Cosynch-56 protocol.
Yayın ingilice ve Fransızcadır. İngilizce özet aşağıdadır.
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In reproductive tissues, GnRH participates in the regulation of cell growth and proliferation by direct binding to the GnRH-R, which is essential for embryo implantation. However, there is no study on the expression and cellular localization of GnRH and GnRH-R in the canine uterus and placenta. Therefore, bitches were ovariohysterectomized 10 to 12 days after mating (vaginal cytology and progesterone measurement), the uteri were flushed, and if embryos were detectable, bitches were allocated to the embryo positive group (E-pos.; preimplantation, n = 5). Other bitches were operated at later stages and, dependent on the gestational age, either allotted to the post-implantation group (Day 18–25 after mating, n = 9), or the mid-gestation group (Day 30–40 after mating, n = 3). Dogs negative in embryo flushing served as controls (E-neg.; controls, n = 5). Samples of the entire uterine wall were taken from the middle of the horn in E-neg. and E-pos. groups, and from placental and interplacental uterine sites in post-implantation and mid-gestation groups. GnRH-R expression was localized at the mRNA and protein levels by immunohistochemistry and in situ hybridization. The expression of GnRH and GnRH-R mRNA was assessed by semiquantitative polymerase chain reaction. Additionally, both GnRH and GnRH-R mRNA were expressed in all tissues examined until mid-gestation. Relative expression of GnRH was higher than that of GnRH-R (P < 0.05). During the post-implantation stage, GnRH-R expression was significantly higher in uteroplacental than in interplacental tissues. In the uterus, GnRH-R stained strongly in the surface and glandular epithelial cells, and seemed to be weaker in myometrium and stroma. Placental signals were predominantly localized in fetal trophoblast cells and to a lesser extent in maternal decidual cells. These findings suggest a local regulatory function of GnRH during early canine pregnancy.
Yayın ingilizcedir.
In reproductive tissues, GnRH participates in the regulation of cell growth and proliferation by direct binding tothe GnRH-R, which is essential for embryo implantation. However, there is no study on the expression and cellular localization of GnRH and GnRH-R in the canine uterus and placenta. Therefore, bitches were ovariohysterectomized 10 to 12 days after mating (vaginal cytology and progesterone measurement), the uteri were flushed, and if embryos were detectable, bitches were allocated to the embryo positive group (E-pos.; preimplantation, n=5). Other bitches were operated at later stages and, dependent on the gestational age, either allotted to the post-implantation group (Day 18–25 after mating,n=9), or the mid-gestation group (Day 30–40 after mating, n=3). Dogs negative in embryo flushing served as controls (E-neg.; controls, n=5). Samples of the entire uterine wall were taken from the middle of the horn in E-neg. and E-pos. groups, and from placental and interplacental uterine sites in post-implantation and mid-gestation groups. GnRH-R expression was localized at the mRNA and protein levels by immunohistochemistry and in situ hybridization. The expression of GnRH and GnRH-RmRNA was assessed by semi-quantitative polymerase chain reaction. Additionally, both GnRH and GnRH-RmRNA were expressed in all tissues examined until mid-gestation. Relative expression of GnRH was higher than that of GnRH-R (P<0.05). During the post-implantation stage, GnRH-R expression was significantly higher in uteroplacental than in interplacental tissues. In the uterus, GnRH-R stained strongly in the surface and glandular epithelial cells, and seemed to be weaker in myometrium and stroma. Placental signals were predominantly localized in fetal trophoblast cells and to a lesser extent in maternal decidual cells. These findings suggest a local regulatory function of GnRH during early canine pregnacy.
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In this study, effect of acupuncture (AP) on the luteal size (LS), luteal blood flow (LBF) and progesterone (P4) levels were investigated in the presence of corpus luteum (CL) in cows. Seven days after 14-days interval PGF2? estrus synchronization protocol, CL positive animals were assigned either to a control group (AP–, n=10) or to an AP group (AP+, n=10) stimulated by using B22 and B23 sensitive acupoints. LS and LBF examinations were carried out before the stimulation (0h) and at 1st, 3rd, 6th hour on d7 and thereafter on d9, d10, d11, d12 and d13 following the AP stimulation in each group with a portable color Doppler ultrasonography. Blood samples for P4 measurement were collected during each examination. There was no significant difference in LS, LBF, or P4 mean values between groups. However, LBF significantly increased at 6h after stimulation (P<0.05) in AP+ group but it increased at d11 in AP– group (P<0.05). The significant increase in LS was observed earlier in AP+ group (on d9; P<0.01) than AP– (on d11; P<0.05). Serum P4 concentrations increased at 3h, d9 and d10 in AP+ group (P<0.05), however a significant difference was only observed at 3h in AP– group (P<0.05). In conclusion, AP stimulation induces earlier increases in LS, LBF and P4 parameters in cows during luteal phase.
Bu çalışmada akupunkturun (AP) ineklerde korpus luteum (CL) varlığında luteal büyüklük (LS), luteal kan akımı (LBF) ve progesteron (P4) seviyeleri üzerindeki etkisi incelendi. 14 gün aralıklı PGF2? östrus senkronizasyon protokolünden 7 gün sonra, CL tespit edilen hayvanlar kontrol (AP–, n=10) ve duyarlı B22 ve B23 kullanılarak stimüle edilen akupunktur gruplarına ayrıldı (AP+, n=10). LS ve LBF incelemeleri taşınabilir bir renkli Doppler ultrasonografi ile 7. günde stimülasyon öncesi (0. saat), izleyen 1, 3, 6. saatler ve 9, 10, 11, 12 ve 13. günlerde yapıldı. Her muayenede P4 ölçümleri için kan örnekleri alındı. Gruplar arasında LS, LBF ve P4 ortalama değerleri farklı değildi. Bununla birlikte, LBF AP+ grubunda stimülasyon sonrası 6. saatte (P<0.05) ancak AP– grubunda 11. günde arttı (P<0.05). LS’deki önemli artış ise AP+ grubunda (9. gün; P<0.01), AP– grubuna göre (11. gün; P<0.05) daha erken gözlendi. Serum P4 konsantrasyonları AP+ grubunda 3. saat, 9. gün ve 10. günde artış gösterdi (P<0.05). Ancak AP– grubunda sadece 3. saatte önemli değişim gözlendi (P<0.05). Sonuç olarak, luteal faz sırasında akupunktur stimülasyonu LS, LBF ve P4 değerlerinde daha erken artışa neden olmaktadır
Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7, 30–45 days after mating), and oocyte positive groups (group 5 [G5], n = 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P < 0.05). In addition, the expression of HIF2A in G5 was higher than that of G1 and HIF2A gene expression at placentation sites of G4 was higher than in G1, G2, and G3 (P < 0.05). Immunohistochemistry indicated that HIF1A, HIF2A, and VEGF expressions were observed in different cell types of uterine and placental tissues in late pregnancy and oocyte groups. The expression of HIF3A did not change significantly in any group investigated. These observations suggest that HIFs and VEGF may play a role in the establishment and development of pregnancy.
Yayın ingilizcedir.
Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7, 30–45 days after mating), and oocyte positive groups (group 5 [G5], n ¼ 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P < 0.05). In addition, the expression of HIF2A in G5 was higher than that of G1 and HIF2A gene expression at placentation sites of G4 was higher than in G1, G2, and G3 (P < 0.05). Immunohistochemistry indicated that HIF1A, HIF2A, and VEGF expressions were observed in different cell types of uterine and placental tissues in late pregnancy and oocyte groups. The expression of HIF3A did not change significantly in any group investigated. These observations suggest that HIFs and VEGF may play a role in the establishment and development of pregnancy
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In the present study, 13 clinical cases of canine mammary adenocarcinoma were evaluated in order to understand the effect of Tarantula cubensis extract (TCE) on tumor tissue. Punch biopsies were taken from the tumors before treatment with TCE. Subcutaneous injections of TCE were administered three times at weekly intervals (3 mL per dog). Between days 7 and 10 after the third injection, the tumor masses were extirpated by complete unilateral mastectomy. Pre- and post-treatment tumor tissues were immunohistochemically assessed. The expression of B-cell lymphoma 2 (Bcl-2) was found to be higher in pre-treatment compared to post-treatment tissues (p < 0.01) whereas Ki-67 expression was lower in post-treatment tissues (p < 0.01). No significant differences in fibroblast growth factor or vascular endothelial growth factor expression were observed between pre- and post-treatment tissues (p > 0.05). The apoptotic index was determined to be low before treatment and increased during treatment. These results suggest that TCE may be effective for controlling the local growth of canine mammary adenocarcinoma by regulating apoptosis.
Yayın ingilzcedir.
Objectives The aim of the study was to evaluate the efficacy of a 4.7 mg deslorelin implant in tom cats. Methods Nine mature male cats were included in the deslorelin group and five cats in the control group. Before the study started, all cats were confirmed to have distinct sexually dimorphic behaviour. Blood samples were taken on the implantation day, at day 7 and at day 15, then monthly, in order to measure serum dihydrotestosterone (DHT) and 17beta(ß)-oestradiol concentrations. The deslorelin group (n = 9) was divided into two subgroups: five cats (cats 1–5) were neutered in the postimplantation period during suppression of sexually dimorphic behaviour, and four cats (cats 6–9) were neutered after re-expression of sexually dimorphic behaviour. The control group cats (n = 5) were castrated without administration of the implant. Results Sexually dimorphic behaviours ceased within a mean ± SD of 13–58 days (23.30 ± 14.17) after implantation. DHT concentration decreased within 30 days. The mean duration of suppression was 26.5 ± 7.42 months and reactivation coincided with increased DHT values reaching preimplantation concentrations within 1 month. 17ß-oestradiol concentrations significantly correlated with DHT concentrations (P <0.01). Castration during suppression of sexual behaviour showed that the length of the long axes of the nuclei of Leydig cells, the diameter of seminiferous tubules and the height of the epithelium of the seminiferous tubules did not change until 3–6 months after implantation, whereas at 12 and 32 months the measured values were even lower than in the control group. In the present study, castration after reactivation showed that the length of long axes of the nuclei of Leydig cells and the diameter of seminiferous tubules approached the values of the control group between 4 and 6 months after reactivation. Conclusions and relevance A deslorelin implant (4.7 mg) suppresses sexually dimorphic behaviour in tom cats without any side effects and with full reversibility; however, duration of suppression is highly individual
Yayın ingilizcedir. İngilizce özet aşağıdadır.
Early pregnancy is one of the most critical periods of pregnancy, and many factors such as cytokines, enzymes, and members of the immune system have to cooperate in a balanced way. In the present study, the gene expression profiles of factors associated with pregnancy such as EGF, transforming growth factor beta, granulocyte-macrophage colonystimulating factor, interferon gamma, insulin-like growth factor 2, insulin-like growth factor 2 receptor, and matrix metalloproteinase 2 were analyzed in uterine tissues of female cats. The cats were assigned to five groups: G1 (embryo positive, n= 7; 7th day after mating), G2 (after implantation, n = 7; 20th day after mating), G3 (midgestation, n = 7; 24–25th day after mating), G4 (late gestation, n =7; 30–45th day after mating), G5 (oocyte group, n = 7; 7th day after estrus). Tissue samples from the uterus and placenta were collected after ovariohysterectomy. Relative messenger RNA levels were determined by real-time polymerase chain reaction. All the factors examined were detected in all tissue samples. In the course of pregnancy, significantly higher expression of EGF and matrix metalloproteinase 2 in G2 than in G1 was observed (P < 0.05). Insulin-like growth factor 2 expression was higher in all groups than in G1 (P < 0.05). Upregulation of EGF during implantation was detected. The expression of interferon gamma was significantly higher in G3 than in G1 (P < 0.05). Transforming growth factor beta and granulocytemacrophage colony-stimulating factor were constantly expressed in all groups. In conclusion, the expressions of these factors in feline uterine tissue at different stages of pregnancy might indicate that these factors play roles in the development of pregnancy such as trophoblast invasion, vascularization, implantation, and placentation.
Yayın ingilizcedir. İngilizce özet aşağıdadır.
Pre-pubertal gonadectomy in dogs and cats is still controver- sially discussed because some consequences cause health problems. Nevertheless, postponement of puberty, that is, prevention of an increase in sexual hormones and thereby prevention of their manifold effects, is of major importance, not only in controlling overpopulation but also to preserve the genetic base for future breeding stock and pets. Therefore, alternatives for surgical suppression of fertility in pre-pubertal animals were critically reviewed. As a promising alternative, the slow-release GnRH agonist deslorelin and other GnRH analogues have been investigated. In female dogs and cats, puberty could be significantly postponed without initial flare- up effect and without disturbance of body development. First trials to delay puberty in female and male cats by application of a 4.7-mg deslorelin implant 24 h after birth so far are promising. In female dogs, a previous investigation showed that when the implant was inserted at the age of 4 months, the initial flare-up effect was prevented. Body development was normal in the studies reviewed here, and with the 9.4-mg implant, puberty was significantly delayed until the age of 21 months or older. In one study, bitches either received a 4.7- or a 9.4-mg implant at the age of 4 months and the epiphyses were mostly closed before the time of first oestrus. Using a 4.7-mg deslorelin implant in pre-pubertal male dogs significantly postponed puberty, and age at puberty was > 2 years when a 9.4-mg implant was used. However, further investigations are required, especially concerning the effect of different GnRH agonist dosages and resorption rates on the duration of postponement of puberty as well as long-term effects in both dogs and cats.
Review ingilizcedir.
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Learning of reproduction
1999: Presentation at the Symposium "Small Animal Reproduction" on the occasion of the 70th birthday of em. Prof. Dr. DDr. h. c. Kurt Arbeiter: “Termination the early pregnanch in the Bitch” 2000- Munich, Maximillian University, Veterinary Faculty (Prof. Stolla) DAAD Scholarship (Research: Blood flow in the corpus luteum in cattle) 2001: Invitation of the Association of Veterinarians for a lecture in Tirol . Reproduction and Neonatology Congress, Alpbach, Tirol. 2008: Hannover, Clinic for Cattle, School of Veterinary Medicine (Prof.Dr. H. Bollwein) DAAD scholarship (Research: Effects of induction of ovulation with GnRH and hCG on follicular and luteal blood flow in Holstein Friesian heifers) 2011-……. Senior Scientist , Project Collaboration : Turkey (with various faculties together) , Vienna, Switzerland Clinic of Obstetrics, Gyneacology/ Institute for Anatomy and Histology 2013-: Invitation of the University of Zurich (Institute of Reproductive and molecular histology and anatomy on): Lecture: Introduction abortion in the bitch: Selecti