Work Experience

  • Todate 2016

    Öğretim Üyesi/ Yardımcı Doçent Doktor /Asst. Prof.

    Yakın Doğu Üniversitesi Veteriner Hekimliği Fakültesi, Mikrobiyoloji Anabilim Dalı

  • 2016 2014

    Öğretim Görevlisi/Doktor/PhD.

    Yakın Doğu Üniversitesi Veteriner Hekimliği Fakültesi, Mikrobiyoloji Anabilim Dalı

  • 2014 2009

    Araştırma Görevlisi/Res.Assist.

    Uludağ Üniversitesi Sağlık Bilimleri Enstitüsü, Veteriner-Mikrobiyoloji Anabilim Dalı

  • 2009 2008

    Doktora Öğrencisi/PhD.Student

    Ankara Üniversitesi Sağlık Bilimleri Enstitüsü /Transfer , Veteriner -Mikrobiyoloji Anabilim Dalı

  • 2008 2003

    Veteriner Hekim/Veterinarian

    Ankara Üniversitesi, Veteriner Fakültesi

Education & Training

  • Ph.D. 2014

    Department of Microbiology, Veterinary Medicine

    Uludag University

  • Master...

    -

    -

  • Bachelor2008

    Faculty of Veterinary Medicine

    Ankara University

Honors, Awards and Grants

Research Projects

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    Investigation of Contagious Agalactia by Bacteriological and Molecular Methods in Sheep and Goats

    The aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balıkesir, Çanakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples,147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate

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    Uterus Bacteriolgy and Effects on Cytology of Pneumovagina in Mares at Bursa Region, Uludag University BAP

    Çalışmamızda Bursa çevresindeki kısraklarda pneumovagina ve ürovaginanın prevalansını, pneumovaginalı kısraklarla fertilite durumlarını, pneumovaginanın uterus bakteriyolojisini ve antimikrobiyal duyarlılığını belirlemek amaçlanmıştır.

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    The phylogenitic typing of Uropathogenic E. coli isolates from cats and dogs and detecting the virulence associated genes in E. coli , Istanbul University, The Scientific Research Project,

    The aim of this study is to perform phylogenetic grouping and detecting the virulence associated genes in E. coli strains isolated from urinary tract infections and to detect the antibiotic susceptibility profiles of these isolates. For this purpose ürine samples will be collected from the cats and dogs with urinary tract infecitons, and the samples will be examined for the occurance of E. coli. Phylogenetic classification of the isolates will be performed according to the apparance of chuA, yjaA ve TSPE4.C2 genes. Then the isolates will be examined for the presence of fimH, saf, afaB/C ,iut fyu, hly , cnf-1, papG virulence factors and the diversity of the papG allelles. Finally the antimicrobial susceptibility profiles of the isolates will be determined.

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Bir Koyun-Keçi Sürüsünde Gözlenen Dermatophilus congolensis İnfeksiyonu

Case Report Uludağ Üniversitesi Veteriner Fakültesi Dergisi, Volume 32, Issue 1, 2013, Pages 2013

Abstract

A case of acute exudative dermatitis was observed in a herd breeding of totally 120 goats and sheeps in Bursa İznik district. The symptoms were observed approximately 70 % of 50 goats and 25 % of 70 sheeps. The lesions generally were located around head parts of animals. Scabs that collected from skin lesions were investigated bacteriologically and mycologically. Giemsa stain was applied and the bacteria was seen as branch-ing filaments containing multiple rows of cocci. No mycologic findings were determined in scabs that applied 10% KOH. The samples were inoculated 5% sheep blood agar and incubated at 37 0C in 5-10% CO2 atmosphere at 24-48 hours for bacteriological examination. While the colonies were formed under of 1 mm diameter after 24 hours, colonies were observed ß-hemolytic, hard, adherent to agar surface and about 1 mm diameter after 48 hours. Gram-positive coccoid forms were seen in Gram stain. Dermatophilus congolensis was identified after bacterioscopy and culture.


Özet

Bursa ili İznik ilçesinde 120 adet koyun ve keçinin birarada yetiştirildiği bir işletmede akut eksudatif der-matitis olgusu gözlendi. Sürüdeki 50 adet keçinin yaklaşık %70’i, 70 adet koyunun ise %25’inde semptomlar gözlendi. Lezyonların genellikle baş bölgesinde olduğu saptandı. Deri lezyonlarından alınan kabuk örnekleri bakteriyolojik ve mikolojik yönden incelendi. Bakteriyoskopide; örneklere Giemsa boyama uygulandı ve ardışık dizili koklar düzensiz, uzun ve büyük filamentler halinde gözlendi. Kabuklarda %10 KOH ile yapılan inceleme-de, mikolojik bulguya rastlanmadı. Bakteriyolojik tanı amacıyla %5 koyun kanlı agara yapılan ekimler 37°C’de, %5-10 CO2 ‘li ortamda 24-48 saat inkübe edildi. İnkübasyon süresi sonunda; ilk 24 saatte çapı 1 mm’nin altında olan ince koloniler, 48 saat sonunda ise yaklaşık 1 mm çapında ß-hemolizli, kuru, agara gömülü koloniler göz-lendi. Gram boyama sonucunda koloniler Gram pozitif kokoid formda görüldü. Bakteriyoskopi ve kültür ince-lemeleri sonucunda yapılan değerlendirmede etken Dermatophilus congolensis olarak identifiye edildi.

Bursa Yenişehir İlçesindeki Bir Keçi İşletmesinde Mycoplasma agalactiae Salgınının Bakteriyolojik ve Serolojik olarak Araştırılması

Conference Paper IX. Ulusal Veteriner Mikrobiyoloji Kongresi, YDÜ,Lefkoşa-KKTC, Volume YDÜ, Issue YDÜ, 2010, Pages YDÜ

Abstract

In this study, 5 joint fluid samples were collected from goats which have shown symptoms of contagious agalactia disease before lactation with no vaccination of agalaxia in a goat management from Bursa Yenişehir district. M. agalactiae was isolated from 4 joint fluid samples. Then,the blood sera were collected from 110 goats and were investigated serologically by ELISA.The 31 of 110 goats were determined as seropositive.Following lactation, second ELISA were applied and seropositivity was determined in 34 goats. M.agalactiae was isolated from 9 of 34 milk samples collected from seropositive goats. High seropositivity were determined by both ELISA in 13 goats in which M.agalactiae had previously been isolated, although 21 goats were also highly seropositive but no agent isolated from their milk samples


Özet

Bursa Yenişehir ilçesinde daha önce agalaksi aşısı yapılmamış bir keçi işletmesinde laktasyondan önce bulaşıcı agalaksi hastalığının semptomlarını gösteren 5 adet keçiden alınan eklem sıvılarının 4’ünden Mycoplasma agalactiae izole edildi. Daha sonra sürüdeki 110 keçiden kan alınarak ELISA testi ile serolojik olarak incelendi ve 31 keçide seropozitiflik belirlendi.Laktasyonu takiben tekrar ELISA testi yapıldı ve 34 keçide seropozitiflik saptandı.Seropozitif keçilerden alınan 34 süt örneğinin 9’undan Mycoplasma agalactiae izole edildi. Mycoplasma agalactiae izole edilen 13 keçide her iki ELISA testinde de yüksek seropozitiflik saptandı,21 keçide ise; ELISA testinde yüksek seropozitiflik saptansa da sütten etken izole edilemedi.

Post-Mortem Evaluation of Gazelles with Pleuropneumonia And Limb Lesions

Conference Paper 61st WDA/10th Biennial EWDA Conference ‘convergence in wildlife health’,Lyon,FRANCE, Volume 61st WDA/10th Biennial EWDA , Issue 61st WDA/10th Biennial EWDA , 2012, Pages 61st WDA/10th Biennial EWDA

Abstract

Necropsy was performed on 12 gazelles which died in 2009-2012 in a local zoo. Animals were coming from various origins, like wildlife or semi-wild conditions like national parks or zoos. Age range was 1-3 years old and 5 were male, 7 were female. Deaths occurred mostly following transportation to indoor enclosures in winter. Extremity wounds healing slowly were the only clinical observation. Interestingly, necropsies of 8 gazelles revealed; severe fibrinopurulent pleuropneumonia which was characterized with necrotic, spheroid, well demarcated lesions of the lungs and fibrinopurulent thoracal effusions. Granulation and scab formation of the limb wounds was remarkable. P. auriginosa in one of these eight gazelles, A. pyogenes in another was isolated from the lungs. Both of the isolated bacteria are opportunistic pathogens causing purulent and rarely systemic infections and rarely reported in gazelles. A. pyogenes is reported to cause necrotizing pneumonia, mandibular osteomyelitis, peritonitis and abscesses in various body parts of some captive wild ruminants, of which gazelles are rarely included. The immunosuppressive effect of the captivity, induction of pulmonary diseases due to population density and indoor housing is considered to play a role in the evaluated gazelles. It reporting this common phenomenon observed in 8 gazelles might be helpful for wildlife health researchers or rehabilitators. Further researches in order to prevent this problem should be investigated.


Özet

Necropsy was performed on 12 gazelles which died in 2009-2012 in a local zoo. Animals were coming from various origins, like wildlife or semi-wild conditions like national parks or zoos. Age range was 1-3 years old and 5 were male, 7 were female. Deaths occurred mostly following transportation to indoor enclosures in winter. Extremity wounds healing slowly were the only clinical observation. Interestingly, necropsies of 8 gazelles revealed; severe fibrinopurulent pleuropneumonia which was characterized with necrotic, spheroid, well demarcated lesions of the lungs and fibrinopurulent thoracal effusions. Granulation and scab formation of the limb wounds was remarkable. P. auriginosa in one of these eight gazelles, A. pyogenes in another was isolated from the lungs. Both of the isolated bacteria are opportunistic pathogens causing purulent and rarely systemic infections and rarely reported in gazelles. A. pyogenes is reported to cause necrotizing pneumonia, mandibular osteomyelitis, peritonitis and abscesses in various body parts of some captive wild ruminants, of which gazelles are rarely included. The immunosuppressive effect of the captivity, induction of pulmonary diseases due to population density and indoor housing is considered to play a role in the evaluated gazelles. It reporting this common phenomenon observed in 8 gazelles might be helpful for wildlife health researchers or rehabilitators. Further researches in order to prevent this problem should be investigated.

Alt Solunum Yolu Hastalıkları Gözlenen Atlarda Bakteriyolojik İncelemeler

Conference Paper X. Ulusal Veteriner Hekimleri Mikrobiyoloji Kongresi (Uluslar arası Katılımlı), Adnan Menderes Üniversitesi-Kuşadası,Aydın, Volume AMÜ, Issue AMÜ, 2012, Pages AMÜ

Abstract

In this study, 66 bronchoalveolar lavage and 3 swap samples were collected from 50 horses and 17 foals with respiratory problems from Bursa and Istanbul Hippodromes. They were investigated bacteriologically for respiratory system infections. The samples were inoculated in 5% sheep blood agar, Tryptic Soy agar, MacConkey agar, Eosin Methylene Blue agar (EMB), modified CAZ-NB agar (mCAZ-NB), Chocolate agar and Edward’s medium. Identification of isolates was done by biochemical tests and API ID Strep Rapid Test Kit. As a result of bacteriological investigation, 50 of 66 bronchoalveolar lavages were found to have 83 bacteria while no bacterium was isolated from 16 samples. 17(20.4%) Staphylococcus spp., 14(16,86%) Streptococcus equi subsp. zooepidemicus, 6(7.2%) Staphylococcus aureus, 5(6.0%) Streptococcus pyogenes, 5(6.0%) Streptococcus dysgalactiae, 5(6.0%) Streptococcus spp., 1(%1.2) Staphylococcus equorum, Streptococcus dysgalactiae subsp. equisimilis, Streptococcus porcinus, Streptococcus agalactiae, Corynebacterium spp. and Rhodococcus equi were identified as Gram positive bacteria. 8(9.6%) Escherichia coli, 2(2.4%) Pseudomonas aeruginosa, 2(2.4%) Pseudomonas alcaligenes, 2 (2.4%) Moraxella spp., 1(1.2%) Actinobacillus spp., Edwardsiella spp., Branhamella spp., Branhamella cuniculi, Neisseria spp., Acinetobacter spp., Klebsiella pneumoniae subsp. pneumoniae , Stenotrophomonas maltophilia , Pseudomonas spp., Enterobacter aerogenes ve Pantoea agglomerans(Enterobacter agglomerans) were identified as Gram negative bacteria. These isolates were used in Kirby-Bauer disk diffusion method to test their antibiotic resistancy patterns. As a result of antibiograms, Gram positive bacteria were mostly found sensitive to Gentamycin, Penicillin G, Rifampicin, Ampicillin, Trimethoprime-Sulfamethoxazole, Erythromycin, Amoxicillin - Clavulanic acid and Poliymyxin B. Gram negative bacteria were found sensitive to Gentamycin, Trimethoprime-Sulfamethoxazole, Ceftiofur, Florfenicol, Enrofloxacine and Tobramycin.


Özet

Bu çalışmada, Bursa ve İstanbul Hipodromlarından solunum yolu semptomu gösteren 50 at ve 17 taydan 66 bronkoalveolar lavaj ile 3 trakeal svap örneği alındı ve solunum sistemi enfeksiyonları yönünden bakteriyolojik olarak incelendi. Toplanan örneklerin %5 koyun kanlı agar, Tryptic Soy agar, Mac Conkey agar, Eosin Methylene Blue agar (EMB), modifiye CAZ-NB agar (mCAZ-NB), Chocolate agar ve Edward’s besiyerlerine ekimleri yapıldı. Ekimler sonucunda üreyen kolonilerin biyokimyasal testler ve API ID Strep Rapid Testi Kiti ile identifikasyonları yapıldı. Bakteriyolojik incelemeler sonucunda 66 bronkoalveolar lavajın 50’sinden 83 adet bakteri izole edilirken 16’sından hiçbir izolasyon yapılmadı. İzole edilen Gram pozitif bakterilerin 17(%20.4) ’si Staphylococcus spp., 14(%16,86) ’ü Streptococcus equi subsp. zooepidemicus, 6(%7.2)’sı Staphylococcus aureus, 5(%6.0)’i Streptococcus pyogenes, 5(% 6.0)’i Streptococcus dysgalactiae, 5(%6.0)’i Streptococcus spp., birer (% 1.2) adedi de Staphylococcus equorum, , Streptococcus dysgalactiae subsp. equisimilis, Streptococcus porcinus, Streptococcus agalactiae, Corynebacterium spp. ve Rhodococcus equi olarak identifiye edildi. Gram negatif bakterilerin 8(% 9.6)’i Escherichia coli, 2(%2.4)’si Pseudomonas aeruginosa, 2(%2.4)’si Pseudomonas alcaligenes, 2(%2.4)’si Moraxella spp., birer (%1.2) adedi de Actinobacillus spp., Edwardsiella spp., Branhamella spp., Branhamella cuniculi, Neisseria spp., Acinetobacter spp., Klebsiella pneumoniae subsp. pneumoniae , Stenotrophomonas maltophilia , Pseudomonas spp., Enterobacter aerogenes ve Pantoea agglomerans (Enterobacter agglomerans) olarak identifiye edildi. İzolatların antibiyogramları Kirby-Bauer Disk Difüzyon metodu ile yapıldı. Antibiyogramlar sonucunda ; Gram pozitif bakterilerin çoğunlukla Gentamisin, Penisilin G, Rifampisin, Ampisilin, Basitrasin, Trimetoprim-Sulfametoksazol, Eritromisin, Amoksisilin-Klavulanik asit ve Polimiksin B’ye karşı duyarlı oldukları ortaya kondu. Gram negatif bakteriler ise Gentamisin, Trimetoprim-Sulfametoksazol, Seftiofur, Florfenikol, Enrofloksasin ve Tobramisin’e karşı duyarlı oldukları ortaya kondu.

Mastitisli Sığır Sütü Kaynaklı S. aureus İzolatlarının Antibiyotik Direnç Profillerinin Saptanması

Conference Paper X. Ulusal Veteriner Hekimleri Mikrobiyoloji Kongresi (Uluslar arası Katılımlı), Adnan Menderes Üniversitesi-Kuşadası, Aydın, Volume AMÜ, Issue AMÜ, 2012, Pages AMÜ

Abstract

Bu çalışmada klinik ve subklinik mastitisli sığır sütlerinden izole edilen S.aureus izolatlarının antibiyotik direnç profillerinin saptanması amaçlandı. Bu kapsamda Bursa ili ve çevresindeki 100 başın üzerindeki süt sığırı işletmelerinden California Mastitis Testi (CMT) ile klinik ve subklinik mastitisli hayvanlar saptandı ve bu hayvanların ilgili meme loblarından steril şartlarda alınan 480 adet süt örneği incelendi. İlgili besiyerlerine yapılan ekimler sonucunda S.aureus şüpheli koloniler API Stapy® ticari biyokimyasal test kiti ile incelendi ve sonuçlar API-Web sistemi ile değerlendirildi. İdentifikasyon sonucunda 480 adet süt örneğinden 151 (%31.45) adet S.aureus izole edildi.. Elde edilen izolatlara 7 farklı gruba ait insan ve hayvanlarda kullanılan 20 çeşit antibiyotik ile Kirby-Bauer Disk difüzyon testi uygulandı ve zon çapları EUCAST Clinical Breakpoint Table Version 2.0, 2012’e göre değerlendirildi . Sonuç olarak klinik ve subklinik mastitis olgularından elde edilen S.aureus izolatlarının, Penisilin grubu antibiyotiklere (Oxacillin, Ampicillin, Penicillin G, Amoxacillin- Clavulanic Acid ve Meticillin) %32.4; Sefalosporinlere (Cephoxitin, Ceftiofour) %38.5; Florokinolonlara (Ciprofloxacin, Ofloxacin, Enrofloxacin) %18.6; Glikopeptid Grubu antibiyotiklere (Vancomycine) %2; Makrolid grubu antibiyotiklere (Azitromisin, Telitromisin, Eritromisin, Klindamisin) %34; Aminoglikozid grubu antibiyotiklere (Gentamisin)%40; Tetrasiklin grubu antibiyotiklere (Tetracycline) %44 ve diğer gruplara da (Mupirocin, Bacitracin, Trimethoprim-Sulphamethaxazole) %10.6 oranında dirençli oldukları saptandı. İzolatlardan 76 (%50.6) adedinin birden fazla antibiyotik grubuna karşı direnç oluşturdukları (Multiple Drug Resistance-MDR) saptandı. S.aureus izolatlarında geniş çaplı antibiyotik direnç profillerinin fenotipik incelenmesi sonucunda yüksek oranda antibiyotik dirençliliği gözlenmiştir. Bu durum gerek hayvan sağlığı gerekse halk sağlığı açısından dikkat çekici niteliktedir.


Özet

Bu çalışmada klinik ve subklinik mastitisli sığır sütlerinden izole edilen S.aureus izolatlarının antibiyotik direnç profillerinin saptanması amaçlandı. Bu kapsamda Bursa ili ve çevresindeki 100 başın üzerindeki süt sığırı işletmelerinden California Mastitis Testi (CMT) ile klinik ve subklinik mastitisli hayvanlar saptandı ve bu hayvanların ilgili meme loblarından steril şartlarda alınan 480 adet süt örneği incelendi. İlgili besiyerlerine yapılan ekimler sonucunda S.aureus şüpheli koloniler API Stapy® ticari biyokimyasal test kiti ile incelendi ve sonuçlar API-Web sistemi ile değerlendirildi. İdentifikasyon sonucunda 480 adet süt örneğinden 151 (%31.45) adet S.aureus izole edildi.. Elde edilen izolatlara 7 farklı gruba ait insan ve hayvanlarda kullanılan 20 çeşit antibiyotik ile Kirby-Bauer Disk difüzyon testi uygulandı ve zon çapları EUCAST Clinical Breakpoint Table Version 2.0, 2012’e göre değerlendirildi . Sonuç olarak klinik ve subklinik mastitis olgularından elde edilen S.aureus izolatlarının, Penisilin grubu antibiyotiklere (Oxacillin, Ampicillin, Penicillin G, Amoxacillin- Clavulanic Acid ve Meticillin) %32.4; Sefalosporinlere (Cephoxitin, Ceftiofour) %38.5; Florokinolonlara (Ciprofloxacin, Ofloxacin, Enrofloxacin) %18.6; Glikopeptid Grubu antibiyotiklere (Vancomycine) %2; Makrolid grubu antibiyotiklere (Azitromisin, Telitromisin, Eritromisin, Klindamisin) %34; Aminoglikozid grubu antibiyotiklere (Gentamisin)%40; Tetrasiklin grubu antibiyotiklere (Tetracycline) %44 ve diğer gruplara da (Mupirocin, Bacitracin, Trimethoprim-Sulphamethaxazole) %10.6 oranında dirençli oldukları saptandı. İzolatlardan 76 (%50.6) adedinin birden fazla antibiyotik grubuna karşı direnç oluşturdukları (Multiple Drug Resistance-MDR) saptandı. S.aureus izolatlarında geniş çaplı antibiyotik direnç profillerinin fenotipik incelenmesi sonucunda yüksek oranda antibiyotik dirençliliği gözlenmiştir. Bu durum gerek hayvan sağlığı gerekse halk sağlığı açısından dikkat çekici niteliktedir.

Bir Muhabbet Kuşu Damızlık İşletmesinde Erysipelas Olgusu

Conference Paper X. Ulusal Veteriner Hekimleri Mikrobiyoloji Kongresi (Uluslar arası Katılımlı),Adnan Menderes Üniversitesi-Kuşadası,Aydın, Volume AMÜ, Issue AMÜ, 2012, Pages AMÜ

Abstract

This study presents Erysipelas case in a parakeet (Melopsittacus undulatus) in a hatchery including 500 Holland, Czech, British breeds located in Bursa-Bandırma district. Ten dead parakeet chicks with the complaint of diarrhea and skin lesions and 30 % mortality, were brought to Uludag University, Faculty of Veterinary Medicine, Departments of Microbiology and Pathology for the diagnostic purposes. At macroscopic and histopathological examination were seen focal disseminated congestive skin areas, mild sero-mucinous rhinitis, focal interstitial pneumonia, congestive liver and increase of bile pigmentation. In the bacteriological investigation, ?-hemolytic, S form colonies were observed at 37 C at 10 % CO2 atmosphere in Colombia agar (5% sheep blood) during 24-48 hours of incubation and after Gram staining of the suspected colonies, Gram positive bacilli were detected. The bacterium was identified as Erysipelothrix rhusiopathiae after routine biochemical tests. Kirby-Bauer disk diffusion method was used for antibiogram, and the isolate found sensitive to Tetracycline (30µg), Gentamycin (10 µg), Ampicillin (10µg ), Penicillin G (10IU); resistant to Enrofloxacine (5µg) phenotypically.


Özet

Bu çalışma, Bursa-Bandırma bölgesinde yer alan ve Hollanda, Çekoslovak, İngiliz ırkı 500 adet damızlık muhabbet kuşu (Melopsittacus undulatus) bulunan bir üretim çiftliğinde saptanan Erysipelas olgusunu sunmaktadır. Uludağ Üniversitesi Veteriner Fakültesi Patoloji ve Mikrobiyoloji Anabilim Dalı’na ilgili damızlık işletmesinden, %30’a yakın yavru ölümleri, diyare ve deri lezyonu bulguları şikayetiyle 10 adet ölü muhabbet kuşu yavrusu, diagnostik amaçla getirildi. Nekropsi materyalleri (akciğer, kalp, karaciğer, bağırsak, deri) ile sinus boşluğundan alınan svaplar ilgili besiyerlerine inokule edildi. Yapılan makroskobik ve histopatolojik inceleme sonucunda deride fokal dissemine konjesyon alanları, burunda hafif sero-musinöz rinitis, akciğerde fokal intersitisyel pnömoni ve karaciğerde konjesyon alanları ve safra pigmenti artışı gözlendi. Bakteriyolojik incelemede ise Colombia agarda (%5 koyun kanlı) 37ºC’de %10 CO2 ‘li ortamda 24-48 saatte, ?-hemolitik S formunda koloniler saptandı ve şüpheli kolonilerden yapılan Gram boyama sonucunda Gram pozitif çomaklar gözlendi. Rutin biyokimyasal testler sonucunda etken Erysipelothrix rhusiopathiae olarak identifiye edildi. İzolat, Kirby-Bauer Disk Difüzyon yöntemi ile yapılan antibiyogramda Tetrasiklin (30 µg), Gentamisin (10 µg), Ampisilin (10µg), Penisilin G (10 IU) duyarlı iken; Enrofloksasin (5µg)’e fenotipik olarak dirençli bulundu.

Bir Kedide Kistik Endometriyal Hiperplazi ve Hidrometra ile Birlikte Her İki Ovaryumda Subsurface Epiteliyal Kistadenom (SES) Olgusu

Conference Paper V.Veteriner Doğum ve Jinekoloji Kongresi, Uludağ Üniversitesi, Antalya, Volume Uludağ Üniversitesi, Issue Uludağ Üniversitesi, 2013, Pages Uludağ Üniversitesi

Abstract

Subsurface epithelial tumors (SES) arise from subsurface structure of ovary. The mean age of affected cats with ovarian tumors is 6.7 years old. SES tumors in dogs and cats may be associated with endometrial hyperplasia. These tumors may be unilateral or bilateral and are often characteristic with papillary and cystic structure with solid areas. In this case, it was aimed to present subsurface epithelial cystadenoma at both ovaries and cystic endometrial hyperplasia with hydrometra in a 13 year-old cross-breed female cat which estrus cycles were suppressed regularly. Both ovaries and cornu uteri were fixed buffered formaline and applied routine histopathological procedures and cut 5 µM thickness, then stained with haematoxylin and eosine (H&E). Fluid samples which was taken from uterus was inoculated general and selective media for bacteriologic isolation and identification. General condition and hematologic findings were normal whereas ultrasound examination of both cornu uteri had mixed tubuler structures containing anechoic cystic areas. Left ovary was normal structure. Macroscopically; at the right ovary had two pieces 0.5 cm in diameter cystic structures were seen and the other ovary had corpus luteum. There was 150 ml clear non-viscous fluid in the uterus. Histopathological examination of cystic ovary, the cyst walls were usually low-cuboidal single layered epithelium structure but in some areas, pseudostratified columnar epithelium structure was observed. Within these cystic structures, papillary protrusions of epithelial cells were seen and there was a homogeneous pink colored fluid in lumen of cyst. Histopathology of the uterus, endometrial glands were cystic and papillary protrusions were seen into the cystic lumen of uterine. Non bacteriologic isolation was detected. Microscopic findings in this case were found to compatible with subsurface epithelial cystadenoma of ovaries and cystic endometrial hyperplasia and hydrometra of uterine.


Özet

Bu olguda 13 yaşlı melez ırkı dişi kediye ait her iki ovaryumda şekillenen subsurface epiteliyal kistadenom ve uterusta oluşan kistik endometriyal hiperplazi ile birlikte hidrometranın incelemesi amaçlanmıştır.

Identification of Mycoplasmas in the preputial swabs of male goats and rams in the Bursa Province of Turkey

Conference Paper International Organisation for Mycoplasmology (IOM) Meeting, İstanbul, TURKEY, Volume IOM, Issue IOM, 2015, Pages IOM

Abstract

The aim of this study was to detect mycoplasmas that cause reproductive infections by testing preputial swabs from rams and male goats. This is the first report that describes the detection of mycoplasma species in preputial swabs from naturally infected rams and male goats in the Bursa Province of Turkey. Bacteriological examination and for the first time 16S rDNA PCR and Denaturing Gradient Gel Electrophoresis (DGGE) was performed on the preputial swabs from Turkish rams and male goats. Both the bacteriological examination and PCR-DGGE method detected two (7.4%) M. bovigenitalium and one (3.7%) M. arginini from a total of 27 samples. M. bovigenitalium and M. arginini were isolated from cases of orchitis in rams and the other M. bovigenitalium was isolated from a male goat with no clinical signs. The PCR-DGGE method was used as it is capable of detecting the diversity of small ruminant mycoplasmas that may be causing reproductive infections. Although culture and PCR/DGGE detected the same mycoplasma species in this study it is a useful test that can be used to monitor samples and prevent the introduction of mycoplasmas onto farms.


Özet

The aim of this study was to detect mycoplasmas that cause reproductive infections by testing preputial swabs from rams and male goats. This is the first report that describes the detection of mycoplasma species in preputial swabs from naturally infected rams and male goats in the Bursa Province of Turkey. Bacteriological examination and for the first time 16S rDNA PCR and Denaturing Gradient Gel Electrophoresis (DGGE) was performed on the preputial swabs from Turkish rams and male goats. Both the bacteriological examination and PCR-DGGE method detected two (7.4%) M. bovigenitalium and one (3.7%) M. arginini from a total of 27 samples. M. bovigenitalium and M. arginini were isolated from cases of orchitis in rams and the other M. bovigenitalium was isolated from a male goat with no clinical signs. The PCR-DGGE method was used as it is capable of detecting the diversity of small ruminant mycoplasmas that may be causing reproductive infections. Although culture and PCR/DGGE detected the same mycoplasma species in this study it is a useful test that can be used to monitor samples and prevent the introduction of mycoplasmas onto farms.

BURSA BÖLGESİNDEKİ PNÖMOVAGİNALI KISRAKLARDA BAKTERİYOLOJİK VE SİTOLOJİK BULGULAR

Conference Paper UMYOS/5th International Vocational Schools Symposium, Volume 1, Issue ., 2016, Pages 379

Abstract

Pneumovagina is the most important cause for the infectious origin infertility in mare. Infectious origin infertility usually results from the bacterial agents. The aim of this study is to determine pneumovagina prevalence and effectivity of the pneumovagina on uterus bacteriology and cytology. A total of 156 mares were evaluated regarding pneumovagina prevalence. Pneumovagina prevalence was determined at 7.1% rate (n=11). In study, group 1 had mares with pneumovagina (n=11), group 2 was control group (n=23). Bacteriologic culture and cytological examination was performed from endometrial swaps taken both groups. Samples taken from group 1 were 100% positive microbiologically. The rate of Escherichia coli, Streptococcus equi subsp. zooepidemicus, Streptococcus equinus and Enterococcus faecium were 40-15.0-10.0%, respectively. Samples taken from the group 2 were 30.4% (n=23) positive microbiologically. Seven positive samples had 14.3% rate Escherichia coli,, 28.5% rate yeast, and 57.2% rate 4 different microorganism. There were intense neutrophils in cytological examinations of mares with pneumovagina. As a result, this study is important to determinate pneumovagina prevalence in mares in Bursa region. Moreover, detection of the intestinal origin bacteria in genital system of the mares with pneumovagina, together with the regular and permanent monitoring studies, performing of the endometrial bacteriology and cytology, it has been considered effective for early stage detection of genital system infections resulted from pneumovagina.


Özet

Pnömovagina, kısraklarda enfeksiyöz orijinli infertilitenin en önemli nedenlerinden biridir. Enfeksiyöz kaynaklı infertilite, çoğunlukla bakteriyel ajanlar tarafından meydana getirilmektedir. Bu çalışmanın amacı, Bursa çevresindeki kısraklarda, pnömovagina prevalansı ve pnömovaginanın uterus bakteriyolojisi ve sitolojisine etkilerinin belirlenmesi amaçlanmıştır. Çalışmada toplam 156 adet kısrak pnömovagina prevalansı yönünden değerlendirilmiştir. Kısraklarda pnömovagina prevalansı %7.1 (n=11) oranında tespit edilmiştir. Çalışmada grup 1’i pnömovaginalı kısraklar (n=11), grup 2’yi ise kontrol grubu (n=23) oluşturmaktadır. Her iki gruptan alınan endometrial svaplardan bakteriyolojik kültür ve sitolojik muayene yapılmıştır. Grup 1’den alınan numunelerin %100’ü (n=11) mikrobiyolojik olarak pozitif bulundu. Numunelerin %40’ı Escherichia coli, %15’i Streptococcus equi subsp. zooepidemicus, %10.0’i Streptococcus equinus ve Enterococcus faecium olarak saptandı. Grup 2’den alınan numunelerin %30.4’ü (n=23) mikrobiyolojik olarak pozitif bulundu. Pozitif bulunan 7 numunenin %14.3’ü Escherichia coli, % 28.5 maya, geri kalan %57.2’si 4 farklı mikroorganizma olarak izole edildi. Pnömovaginalı kısraklardan yapılan sitolojik muayenede yoğun nötrofili belirlendi. Sonuç olarak, bu çalışma Bursa bölgesindeki kısraklarda ilk olarak pnömovagina prevalansının belirlenmesi yönüyle önemlidir. Ayrıca pnömovaginalı kısraklarda barsak orijinli bakterilerin genital sistemde saptanması, pnömovaginanın düzenli ve sürekli monitoring çalışmalarıyla birlikte, endometrial bakteriyoloji ve sitolojinin yapılması, pnömovagina kaynaklı genital sistem enfeksiyonlarının erken belirlenmesinde etkin olacağı kanaatine varılmıştır.

ÜRİNER SİSTEM İNFEKSİYONU SEMPTOMLU BİR KEDİNİN İDRAR KÜLTÜRÜNDEN Trichosporon asahii İZOLASYONU VE İDENTİFİKASYONU

Conference Paper XII. Veteriner Hekimleri Mikrobiyoloji Kongresi, Volume ., Issue ., 2016, Pages 173-174

Abstract

: In this case, bacteriological and mycological examination findings of urine sample collected by cystocentesis from a cat with urinary tract infection symptom is described. The isolates were obtained from urine sample of a cat with polyuria complaint which was brought to the Animal Hospital, The Faculty of Veterinary Medicine, Uludağ University. For the bacteriological identification, the sample was plated to 5% sheep blood agar, MacConkey agar and Eosin Methylene Blue (EMB) agar, and incubated for 24-48 hours at 37 °C in aerobic and 35 °C in microaerophilic conditions. Also the urine was centrifuged at 3000 rpm for 20 min, the supernatant was discarded and the sediment was plated in the same manner as described above. The colonies were identified according to their Gram staining properties and biochemical activities. Identification of the isolate was confirmed by using VITEK 2 Compact System (Biomerieux, France) in the Microbiology Laboratory of Faculty of Veterinary Medicine, Near East University. For the mycological examination, the urine sample was inoculated onto Sabouraud Dextrose agar (SDA) and incubated at 28 °C and 37 °C for 7 days. Gram staining properties of the colonies were evaluated and mycelial colonies were stained with Lactophenol Cotton Blue and examined microscopically. The isolate was identified by using biochemical tests and API 20C AUX system kit (Biomerieux, France). In this case, as a result of bacteriological and mycological examinations, Trichosporon asahii, Aerococcus viridans and Aspergillus spp. were isolated and identified. This study also reports the first case of urinary tract infection in a male cat caused by Trichosporon asahii.


Özet

Bu olguda; üriner sistem infeksiyonu semptomlu bir kediden sistosentez yöntemiyle alınan idrar örneğinde bakteriyolojik ve mikolojik bulgular ortaya konulmuştur. Uludağ Üniversitesi Veteriner Fakültesi Hayvan Hastanesine poliüri şikayeti ile getirilen bir kedinin idrar örneğinden bakteriyolojik tanı için % 5 koyun kanlı agara, MacConkey agara ve Eosin Methylene Blue (EMB) agara ekimleri yapılarak 37 °C’de aerobik ortamda ve 35 °C’de mikroaerofilik ortamda 24-48 saat süreyle inkübe edildi. Ayrıca 20 dk 3000 rpm’de santrifüj edilen idrardan santrifüj sonrası süpernatant atılarak dipte oluşan tortudan ekim yapıldı. Ekimler sonucunda şekillenen kolonilerin Gram boyanma özellikleri ve biyokimyasal testler ile identifikasyonları yapıldı. Elde edilen izolatın, Yakın Doğu Üniversitesi Veteriner Fakültesi Mikrobiyoloji Laboratuvarı’nda VITEK 2 Compact Sistem (Biomerieux, Fransa) cihazı kullanılarak identifikasyonu konfirme edildi. Mikolojik muayene için idrar örneği Sabouraud Dextrose agara (SDA) ekimi yapılarak 28 °C’de ve 37 °C’de 7 gün süreyle inkübe edildi. Şekillenen kolonilerin Gram boyama özellikleri değerlendirildi ve miselyal formdaki mantar kolonileri Laktofenol Pamuk Mavisi ile boyanarak mikroskopta incelendi. Biyokimyasal testler ve API 20C AUX sistem kiti (Biomerieux, Fransa) kullanılarak identifikasyonu yapıldı. Bu vakada, bakteriyolojik ve mikolojik muayeneler sonucunda Trichosporon asahii, Aerococcus viridans ve Aspergillus spp. izole ve identifiye edilmiştir. Bu olgu, aynı zamanda erkek bir kedide görülen Trichosporon asahii’nin neden olduğu ilk üriner sistem infeksiyonu olgusudur.

Marmara Bölgesinde Koyun ve KEçilerde Mycoplasma agalactiae'nın Bakteriyolojik ve Moleküler Yöntemler ile Araştırılması

Conference Paper XII. Veteriner Hekimleri Mikrobiyoloji Kongresi, Volume ., Issue ., 2016, Pages 89-90

Abstract

The aim of this sudy was diagnosis that occurence of the main agent ‘Mycoplasma agalactiae (Ma)’ of Contagious Agalactia by bacteriological and molecular methods from sheep and goats in Marmara Region.A total of 339 samples from 162 milk samples,147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue samples were examined by bacteriological and molecular methods for sheep and goats in Bursa, Balıkesir, Çanakkale and Edirne provinces. The 339 samples were collected from 190 adult female goats, 57 ewes, 19 young female goats, 6 male goats, 27 lambs and 6 rams. Among 29 (8.5%) Mycoplasma sp. isolates; 25 (86.20%) were identified as Mycoplasma agalactiae, 2 (6.89%) as Mycoplasma ovipneumoniae and 2 (6.89%) as Mycoplasma arginini.In molecular diagnosis, the 234 samples were analysed using PCR protocols which uvrC gene PCR results could be detected Mycoplasma agalactiae positive with 9.4 % rate. On the other hand, polC gene-PCR detected 13.24% rate. The samples were analysed by uvrC-PCR and detected Mycoplasma agalactiae positive with 15.74% rate of milk samples, 6.66% rate of joint fluids and 25% rate of lung tissue samples in goats. The result of polC-PCR detected Mycoplasma agalactiae positive with 22.04% rate of milk samples, 6.66% rate of joint fluids, 1.20% rate of eye swabs and 25% rate of lung tissue samples in goats. When compare between bacteriological examination and PCR, M.agalactiae was detected by 10.68% rate. As a result of this, 7 milk samples and 2 eye swabs were detected positive by polC-PCR while negative by bacteriological examination and uvrC –PCR. As a result of this polC-PCR protocol was to high rate (13.24%) of detection of Mycoplasma agalactiae when it was compared to uvrC-PCR protocol. In conclusion, presence of Contagious Agalactia in Marmara region was investigated by bacteriological and molecular methods and Mycoplasma agalactiae was diagnosed as primary cause of Contagious Agalactia.


Özet

Bu çalışmanın amacı, Marmara Bölgesi illerine ait koyun ve keçilerde Bulaşıcı Agalaksi hastalığının başlıca etkeni olan Mycoplasma agalactiae (Ma)’nın varlığını bakteriyolojik ve moleküler yöntemler ile teşhis etmektir. Çalışmada; Bursa, Balıkesir, Çanakkale ve Edirne illerindeki koyun ve keçilerden 162 adet süt örneği, 147 adet göz svabı, 15 adet eklem sıvısı, 11 adet burun svabı ve 4 adet akciğer dokusu olmak üzere alınan toplam 339 örnek bakteriyolojik ve moleküler yöntemlerle incelenmiştir. 339 örneğin 190’ı keçi, 57’si koyun, 19’u oğlak, 6’sı teke, 27’si kuzu ve 6’sı koç’a ait numunelerdir. Bakteriyolojik incelemede uygulanan biyokimyasal testler ve üreme inhibisyon testleri sonucunda, Mycoplasma sp. olarak değerlendirilen 29 (%8.5) izolatın 25’i Mycoplasma agalactiae (%86.20) olarak identifiye edildi. Sadece biyokimyasal test sonuçlarına göre 2 izolat Mycoplasma ovipneumoniae (%6.89) ve 2 izolat Mycoplasma arginini (%6.89) olarak identifiye edildi. Moleküler teşhiste, 234 örneğe uygulanan PCR protokolleri ile Mycoplasma agalactiae uvrC geni PCR sonuçlarında %9.4 oranında Mycoplasma agalactiae pozitif tespit edilirken, polC geni ile yapılan PCR sonucunda %13.24 oranında Mycoplasma agalactiae pozitif bulundu. Uygulanan uvrC-PCR sonuçlarına göre, süt örnekleri %15.74 oranı ile, eklem sıvı örnekleri %6.66 oranı ile ve akciğer örnekleri %25 oranı ile pozitif bulundu. PolC -PCR sonuçlarına gore ise, süt örnekleri %22.04 oranı ile, eklem sıvı örnekleri %6.66 oranı ile, göz svabı örnekleri %1.20 oranı ile ve akciğer örnekleri %25 oranı ile pozitif bulundu. PCR sonuçları ve bakteriyolojik incelemeler değerlendirildiğinde %10.68 oranında M.agalactiae identifiye edildi. Aynı zamanda 7 keçi sütünde ve 2 göz svabında bakteriyolojik incelemelerde ve uvrC- PCR’da Mycoplasma agalactiae negatif iken, polC -PCR sonucunda bu örnekler pozitif bulundu. PCR sonuçlarına göre, Mycoplasma agalactiae’nın deteksiyonunda % 13.24 oranı ile polC-PCR metodunun diğer metodlara göre daha fazla deteksiyon sağladığı gözlendi. Sonuç olarak, Marmara bölgesinde Bulaşıcı Agalaksi hastalığının varlığı bakteriyolojik ve moleküler yöntemler ile araştırıldı ve hastalığa neden olan başlıca etkenin M.agalactiae olduğu tespit edildi.

http://www.cabdirect.org/abstracts/20143091538.html

General Publication Dermatophilus congolensis infection in a sheep-goat herd., 2016

Abstract


Özet

http://www.cabdirect.org/abstracts/20153076313.html

General Publication Bacteriological and cytological findings of bronchoalveolar lavage fluids in foals with lower respiratory tract diseases., 2016

Abstract


Özet

https://www.researchgate.net/profile/Esra_Buyukcangaz/publication/230665930_Molecular_characterisation_of_quinolone_resistance_in_Escherichia_coli_from_animals_in_Turkey/links/5539342d0cf2239f4e7d8d1d.pdf

General Publication Molecular characterisation of quinolone resistance in Escherichia coli from animals in Turkey, 2016

Abstract


Özet

http://www.cabdirect.org/abstracts/20143402014.html

General Publication Investigation of Contagious Agalactia by bacteriological and PCR methods in sheep and goats., 2016

Abstract


Özet

http://veterinaryrecord.bmj.com/content/171/23/597.2.short

General Publication Effects of levamisole and ranitidine on antibody-forming responses induced by killed Mycoplasma vaccine antigens in Saanen goats, 2016

Abstract


Özet

http://journals.tubitak.gov.tr/veterinary/abstract.htm?id=19228

General Publication Comparison of PCR tests for the detection of Mycoplasma agalactiae in sheep and goats, 2016

Abstract


Özet

http://www.cabdirect.org/abstracts/20113405610.html

General Publication Evaluation of effectiveness of tylosin in a goat herd naturally suffering from Mycoplasma agalactiae., 2016

Abstract


Özet

Evaluation of effectiveness of tylosin in a goat herd naturally suffering from Mycoplasma agalactiae

Original Article Uludağ Üniversitesi Veteriner Fakültesi Dergisi, Volume 32, Issue 1, 2011, Pages 13-16

Abstract

In this study, a goat herd with Contagious Agalactia symptoms was investigated bacteriologically and serologically and evaluated the excreation of the agent after tylosin treatment. In the serological studies, 110 goat sera were investigated by ELISA and 31 of them were found seropositive. In the clinically examination of seropositive goats, it was determined that 10 goats had been shown syptoms of Contagious Agalactia. Joint swelling was determined in 5 of 10 goats and lameness was determined in the other 5 goats without joint swelling. Joint fluids of 5 goats with joint swelling were investigated bacteriologically and Mycoplasma agalactiae was isolated from 4 of them. Tylosin® (Tylan®, Lilly Elanco, Türkiye) treatment was performed on these 10 clinically ill goats at the dose of 10 mg/kg throughout five days. Following lactation, second ELISA were applied and seropositivity was detected in 34 goats. M.agalactiae was isolated from 9 of 34 milk samples collected from seropositive goats. One of the Tylosin treated goats were detected to excreate of the agent with milk while no M.agalactiae were isolated the others milk samples. As a consequence, These results were estimated that Tylosin treatment may reduce excreation of M.agalactiae with milk after lactation and have favourable effects on the recovery of the clinical symptoms caused by Contagious agalactia.


Özet

Bu çalışmada, Bursa Yenişehir’de Bulaşıcı Agalaksiya hastalığı semptomları gösteren bir keçi sürüsü bakteriyolojik ve serolojik olarak incelendi ve Tylosin tedavisi sonrasında etken saçılımı değerlendirildi. Bütün hayvanların klinik muayenelerinin yapılmasının ardından serolojik muayeneleri yapıldı. Klinik muayene sonucunda 10 adet keçinin Bulaşıcı Agalaksiya hastalığının semptomlarını gösterdiği tespit edildi. Çalışmalarda 110 keçinin kan serumları ELISA ile incelendi ve klinik bulgu gösteren 10 hayvan dâhil 31 keçide seropozitiflik belirlendi. Bu 10 keçinin beşinde eklem şişlikleri, diğer beşinde ise eklem şişliği olmaksızın topallık gözlendi. Eklemlerinde şişlik bulunan beş keçiden alınan eklem sıvıları bakteriyolojik olarak incelendi ve dördünden Mycoplasma agalactiae izole edildi. Semptom gösteren 10 keçiye 5 gün süre ile 10 mg/kg Tylosin® (Tylan®, Lilly Elanco, Türkiye) uygulaması yapıldı. Laktasyonu takiben bütün keçilere tekrar ELISA testi yapıldı ve 34 keçide seropozitiflik saptandı. Seropozitif keçilerden alınan 34 süt örneğinin 9’undan Mycoplasma agalactiae izole edildi. Tylosin tedavisi uygulanan 10 keçiden sadece birisinde süt ile etken saçıldığı saptandı. Sonuç olarak, Tylosin uygulamalarının M. agalactiae’nın laktasyon sonrasında süt ile saçılımını azaltması ve klinik bulgularının iyileşmesi yönünde olumlu etkilerinin olduğu kanısına varıldı.

Molecular characterisation of quinolone resistance in Escherichia coli from animals in Turkey

Original Article Veterinary Record, Volume doi: 10.1136/vr.100719, Issue doi: 10.1136/vr.100719, 2012, Pages doi: 10.1136/vr.100719

Abstract

The aim of this study were to detect the gyrA, parC and marR mutations and qnr genes (qnrA, qnrB and qnrS) in 120 strains of Escherichia coli isolated from animals. European Committee on Antimicrobial Susceptibility Testing and Clinical Laboratory Standards Institute disc diffusion and minimum inhibitory concentration (MIC) tests, respectively, were used to determine fluoroquinolone (FQ) resistance, and molecular methods were used to detect the mutations and the genes. E coli isolates with an MIC of ?8 mg/l had mutation at Ser-80 in parC in addition to mutations at Ser-83, Asp-87 or both in gyrA. The nucleotide change was detected in marR (Ser-3 › Asn, Ala-53 › Glu, Gly-103 › Ser, Tyr-137 › His). Only four E coli isolates (3.3 per cent) contained qnrA and qnrS, and qnrB was not detected. Two E coli isolates from healthy calves also contained qnrA and qnrS. The MICs of enrofloxacin and danofloxacin for qnr-containing E coli isolates ranged from 32 mg/l to 256 mg/l. The results of this study indicated that the FQ-resistant E coli isolates presented an alteration in gyrA (Ser-83 › Leu, Asp-87 › Asn) and parC (Ser-80 › Ile) with high MICs (8–256 mg/l), and there was a low prevalence of qnr genes among E coli isolated from animals.


Özet

The aim of this study were to detect the gyrA, parC and marR mutations and qnr genes (qnrA, qnrB and qnrS) in 120 strains of Escherichia coli isolated from animals. European Committee on Antimicrobial Susceptibility Testing and Clinical Laboratory Standards Institute disc diffusion and minimum inhibitory concentration (MIC) tests, respectively, were used to determine fluoroquinolone (FQ) resistance, and molecular methods were used to detect the mutations and the genes. E coli isolates with an MIC of ?8 mg/l had mutation at Ser-80 in parC in addition to mutations at Ser-83, Asp-87 or both in gyrA. The nucleotide change was detected in marR (Ser-3 › Asn, Ala-53 › Glu, Gly-103 › Ser, Tyr-137 › His). Only four E coli isolates (3.3 per cent) contained qnrA and qnrS, and qnrB was not detected. Two E coli isolates from healthy calves also contained qnrA and qnrS. The MICs of enrofloxacin and danofloxacin for qnr-containing E coli isolates ranged from 32 mg/l to 256 mg/l. The results of this study indicated that the FQ-resistant E coli isolates presented an alteration in gyrA (Ser-83 › Leu, Asp-87 › Asn) and parC (Ser-80 › Ile) with high MICs (8–256 mg/l), and there was a low prevalence of qnr genes among E coli isolated from animals.

Effects of levamisole and ranitidine on antibody forming responses induced by killed Mycoplasma vaccine antigens in Saanen goats

Original Article Veterinary Record, Volume doi:10.1136/vr.101098,171:589, Issue doi:10.1136/vr.101098,171:589, 2012, Pages doi:10.1136/vr.101098,171:589

Abstract

This study demonstrates that levamisole and ranitidine have immunomodulatory effects in goats following vaccination by killed M. agalactia vaccine. When combined with vaccination, administration of these drugs is a useful and cheap method to boost immunity in goats. Further studies of different administration regimens are required to determine the most appropriate doses and administration timing for levamisole and ranitidine.


Özet

This study demonstrates that levamisole and ranitidine have immunomodulatory effects in goats following vaccination by killed M. agalactia vaccine. When combined with vaccination, administration of these drugs is a useful and cheap method to boost immunity in goats. Further studies of different administration regimens are required to determine the most appropriate doses and administration timing for levamisole and ranitidine.

Koyun ve Keçilerde Bulaşıcı Agalaksi Hastalığının Bakteriyolojik ve PCR Metotları ile Araştırılması

Original Article Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Volume 21, Issue 1, 2014, Pages 75-80

Abstract

The aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balıkesir, Çanakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples,147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.


Özet

Bu çalışmada, koyun ve keçilerde Bulaşıcı Agalaksi hastalığının varlığını bakteriyolojik ve moleküler yöntemler ile teşhis etmek amaçlandı. Bursa, Balıkesir, Çanakkale ve Edirne illerine ait koyun ve keçilerden toplanan 339 adet örnek bakteriyolojik ve moleküler yöntemlerle incelendi. Örneklerin 162 adedini süt örneği, 147 adedini göz svabı, 15 adedini eklem sıvısı, 11 adedini burun svabı ve 4 adedini de akciğer dokusu oluşturmuştur. Bakteriyolojik incelemede 29 izolat Mycoplasma sp. olarak değerlendirildi. Uygulanan biyokimyasal testler ve üreme inhibisyon testleri sonucunda, 29 (%8.55) izolatın 25’i (%7.37) Mycoplasma agalactiae olarak, 2 (%0.58)’si Mycoplasma ovipneumoniae ve 2 (%0.58)’si de Mycoplasma arginini olarak identifiye edildi. Moleküler teşhiste ise, polC-PCR sonucunda %9.14 oranında M. agalactiae pozitif bulundu. PCR bulguları ile bakteriyolojik bulgular karşılaştırıldığında, 5 süt örneği ve 1 akciğer örneği polC-PCR ile M. agalactiae pozitif bulunurken, kültür ile negatif bulundu. PolC-PCR sonuçlarına göre, süt örnekleri %14.19 oranı ile, eklem sıvı örnekleri %13.33 oranı ile, göz svabı örnekleri %2.72 oranı ile ve akciğer örnekleri %50 oranı ile pozitif bulunurken, burun svabı örnekleri negatif bulundu. Bu çalışmada, Bulaşıcı Agalaksi hastalığının varlığı bakteriyolojik ve moleküler yöntemler ile araştırılmış ve başlıca hastalığa neden olan etkenin M. agalactiae olduğu tespit edilmiştir, hastalığa neden olan diğer mikoplazma etkenlerine rastlanılmamıştır.

Bacteriological and Cytological Findings of Bronchoalveolar Lavage Fluids in Foals with Lower Respiratory Tract Diseases

Original Article Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Volume 21, Issue 3, 2015, Pages 355-359

Abstract

Lower respiratory tract disease is one of the most common causes of economic loss in foals. There are several methods used for the diagnosis of this disease. Bronchoalveolar lavage is one of the most important technique for bacteriological and cytological examination. The aim of this study was to evaluate BAL samples’ cytologic and bacteriologic examination of foals with lower respiratory tract disease. In the comparison of cytologic examination of BAL samples with bacterial agents and without bacterial agents statistical differences in the percentage of neutophils, macrophages (P<0.001) and lymphocytes (P<0.05) were defined. Also according to the bacteriologic examination results of the BAL samples in 20 foals following agents were mainly detected: Streptococcus equi subsp. zooepidemicus (10), Staphylococcus aereus (7), Escherichia coli (3). Totally 25 aerobic/facultative gram (+) and gram (-) bacils were isolated. In conclusion, the BAL technic is useful for cytological and bacteriological examination in horses with lower respiratory tract diseases


Özet

Taylarda alt solunum sistemi hastalıkları at yetiştiriciliğinde ekonomik kayıpların başında gelmektedir. Bu hastalıkta etkenin kesin tanısı için kullanılan birçok yöntem bulunmaktadır. Bronkoalveolar lavaj, hem bakteriyel hem de sitolojik muayenenin yapılabilmesi açısından en önemli tekniklerden biridir. Bu çalışmada, alt solunum sistemi hastalığı olan taylarda BAL örneklerinin sitolojik ve bakteriyolojik muayene bulgularının değerlendirilmesi amaçlandı. BAL örneklerinin sitolojik muayenelerine göre etken üreyen olgularda etken üremeyen olgulara göre nötrofil, makrofaj (P<0.001) ve lenfosit hücrelerinin (P<0.05) yüzde oranları arasında istatistiki olarak önem bulundu. Ayrıca alınan BAL sıvısından yapılan bakteriyolojik muayene sonucunda 20 tayda, Streptococcus equi subsp. zooepidemicus (10), Staphylococcus aereus (7), Escherichia coli (3), etkenleri çoğunlukta olmak üzere toplamda 25 adet aerobik/fakültatik gram (+) kok ve gram (-) basil grubunda bakteri üremesi tespit edildi. Sonuç olarak, alt solunum yolları hastalıklarında, özellikle endoskopi eşliğinde yapılan BAL tekniğinin gerek sitolojik değerlendirme, gerekse bakteriyolojik muayene amacıyla uygulanmasının tanı ve tedavi protokolünün oluşturulması açısından büyük önem taşıdığı sonucuna varıldı.

Bacteriologic and Cytologic Examination Results of Mares with Pneumovagina in Bursa Region

Original Article International Journal of Veterinary Science, Volume 5, Issue 4, 2016, Pages 295-298

Abstract

Pneumovagina is one of the most important causes for bacteria origin infectious infertility in mares. The aim of this study was to determine side effects of the pneumovagina on uterus bacteriology and cytology and its prevalence in Bursa region. A total of 156 mares were evaluated regarding to prevalence of pneumovagina, and bacteriologic and cytologic examination results. Group I had 11 mares with pneumovagina (n=11), and group II was control group including 23 mares (n=23). Bacteriologic culture and cytological examination were performed from samples taken by endometrial swabs in both groups. The prevalence of pneumovagina was 7.1% (n=11). Microbiologically, group I had 100% positive culture results. The rate of Escherichia coli, Streptococcus equi subsp. zooepidemicus, Streptococcus equinus and Enterococcus faecium were 40.0-15.0-10.0%, respectively. Group II had 30.4% (n=23) positive results. Seven of them were including 14.3% rate of Escherichia coli, 28.5% rate of yeasts, and 57.2% rate of 4 different microorganisms. There were intense neutrophils in cytological preparations of group I. Antimicrobial susceptibility was performed in both groups’ microorganisms and penicillin, cefquinom, marbofloxacin tetracycline, erythromycin, florfenicol Ceftiofur amoxicillin, amoxicillin / clavulanic acid, enrofloxacin, gentamicin gentamicin colistin and Sulfamethoxazole/Trimethoprimwere selected as antibiotics for this test. Isolates were much more resistant to tetracycline, erythromycin, gentamicin, colistin and sulfamethoxazole/trimethoprim. As a conclusion, it has been emphasized that examination of endometrial bacteriology and cytology in mares with pneumovagina should be done to determinate early stage genital system infections


Özet

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ISOLATION OF STREPTOCOCCI FROM A FATAL CASE OF MYOCARDITIS IN A CAPTIVE BROWN BEAR (URSUS ARCTOS)

Original Article Journal of Zoo and Wildlife Medicine , Volume ., Issue ., 2016, Pages .

Abstract

A 10-year-old, male brown bear (Ursus arctos) died without any apparent signs in Bursa Zoo from Turkey. Severe purulent pericarditis and myocarditis with mild ascites, lung edema and moderate liver congestion were observed during necropsy. Microscopically, there were severe neutrophilic infiltrations in myocardium and thoracic lymph nodes. Streptococcus bovis type II/4 was identified phenotypically from the heart. Because of the discrepancy in taxonomy of Streptococci, the agent was classified as a member of the Streptococcus bovis/Streptococcus equinus complex (SBSEC).


Özet

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DETERMINATION OF MILK COMPOSITION, BACTERIOLOGY AND SELECTED BLOOD PARAMETERS OF DAIRY GOATS UNDER DIFFERENT FEEDING SYSTEMS

Original Article Animal Review /PAK Publishing, Volume 3, Issue 2, 2016, Pages 36-42

Abstract

This research was carried out at two farms located in the Karacabey region of Turkey: an extensive goat farm (A) and a semi-intensive goat farm (B). A total of 32 Saanen goats (3 years old) at an early stage of their second lactation were selected from Farm A and Farm B. The total DM intake (TDM) values were 1.89 and 1.86 (kg d-1) for goats housed on the A and B farms, respectively. Compared with Farm A, Farm B produced more milk each day (P<0.05; 1.38 -. 1.76 kg day-1). The milk samples taken from Farm A had a higher (P<0.05) milk fat content than the samples from Farm B (milk fat=4.40 and 1.89 %, respectively). The serum creatinine values were significantly higher (P<0.05) in the blood of goats from farm A compared with farm B (1.11 and 0.56 mg dl-1, respectively). Comparison of glucose levels from both farms showed a significantly higher level of glucose in the blood samples from goats at Farm B (P<0.05; 24.23 and 61.43 mg dl-1). Serum parameters for cholesterol, GGT and urea were not affected by the feeding system (P > 0.05).


Özet

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Comparison of PCR tests for the detection of Mycoplasma agalactiae in sheep and goats

Original Article Turkish Journal of Veterinary and Animal Sciences (Turk J VetAnim Sci), Volume DOI: 10.3906/vet-1511-65 , Issue DOI: 10.3906/vet-1511-65 , 2016, Pages DOI: 10.3906/vet-1511-65

Abstract

The aim of this study was to compare PCR tests for the detection of Mycoplasma agalactiae in sheep and goats. Samples obtained from sheep and goats in Turkey were tested to determine the presence of M. agalactiae. Mycoplasma culture and two direct PCR methods based on the uvrC gene and polC gene were compared with PCR of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (PCR/DGGE) on DNA extracted from clinical samples and following culture enrichment. A total of 234 samples were examined, 9.4% and 13.2% were positive for M. agalactiae based on the uvrC gene and polC gene PCRs, respectively, while the culture method revealed that 12.8% of the samples were mycoplasma-positive. The PCR/DGGE method identified M. agalactiae in 15.0% of the samples; it also detected and identified other Mycoplasma species in the samples. M. agalactiae was only detected in goats in this study. This is the first time 16S rRNA PCR/DGGE was performed on various samples from sheep and goats in Turkey. This study confirmed that M. agalactiae and other Mycoplasma species are present in goats indicating a requirement for effective control strategies for Contagious Agalactia and Mycoplasma species in Turkey.


Özet

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    MICROBIOLOGY II

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    EPIDEMIC AND ZOONOTIC DISEASES AND PREVENTATION

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Teaching History

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    VETERINARY IMMUNOLOGY I

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    VETERINARY IMMUNOLOGY

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  • 2016 GÜZ

    MICROBIOLOGY I

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    MICROBIOLOGY I

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    POULTRY DISEASES

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    MICROBIOLOGY II

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  • 2018 BAHAR

    MICROBIOLOGY II

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    IMMUNOLOGY

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  • 2017 GÜZ

    MICROBIOLOGY

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    IMMUNOPROPHYLAXIS

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    THESIS

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  • 2017 GÜZ

    POULTRY DISEASES

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    POULTRY DISEASES

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    POULTRY DISEASES

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  • 2019 GÜZ

    MICROBIOLOGY

  • 2017 BAHAR

    EPIDEMIC AND ZOONOTIC DISEASES AND PREVENTATION

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  • 2018 BAHAR

    EPIDEMIC AND ZOONOTIC DISEASES AND PREVENTATION

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  • 2017 GÜZ

    POULTRY DISEASES

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  • 2015 GÜZ

    MICROBIOLOGY

  • 2017 BAHAR

    MICROBIOLOGY II

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  • 2018 BAHAR

    MICROBIOLOGY II

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  • 2016 GÜZ

    MICROBIOLOGY

  • 2015 BAHAR

    EPIDEMIC AND ZOONOTIC DISEASES AND PREVENTATION

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  • 2018 GÜZ

    MICROBIOLOGY

  • 2015 BAHAR

    MICROBIOLOGY II

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  • 2016 BAHAR

    EPIDEMIC AND ZOONOTIC DISEASES AND PREVENTATION

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