Work Experience

  • Todate 2012

    Öğretim Üyesi

    Yakın Doğu Üniversitesi, Biyokimya

Education & Training

  • Ph.D. 2012

    Biyokimya

    Yakın Doğu Üniversitesi

  • Master2008

    Genetik

    İstanbul Üniversitesi

  • Bachelor2005

    Tıbbi Biyolojik Bilimler

    İstanbul Üniversitesi

Honors, Awards and Grants

  • 2015
    DESAM, Genç Araştırmacı Ödülü
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  • 2016
    DESAM, Genç Araştırmacı Ödülü
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  • 2015
    DESAM, Bilimsel Başarı Ödülü
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Research Projects

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    Molohiya (Corchorus Olitorius) Ektraktının Kolon Kanseri Üzerine Apoptotik Etkileri (yardımcı araştırmacı) (2016-halen devam ediyor)

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    Nijerya’da Halk Arasında Kansere Karşı Kullanılan Bazı Bitkilerin Antikanser Aktivitelerinin Araştırılması (yardımcı araştırmacı)(2016-halen devam ediyor)

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    Humik Asitin Gastrik Adenokarsinoma Hücre Hattında Sitotoksitite Üzerine Etkisinin Araştırılması (yardımcı araştırmacı)(2016- halen devam ediyor)

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Serum Leptin Level and Lipid Profile in Childhood Obesity

Conference Paper Turkish Journal of Biochemistry, Volume 37, Issue 1, 2012, Pages 210

Abstract

Obesity is a growing worldwide health problem affecting both adults and children. Childhood obesity can increase the risk of health problems such as insulin resistance and metabolic syndrome, as well is a risk for the future development of adult obesity or cardiovascular disease. Nonetheless, information regarding childhood obesity and its relationship to adipocytokines in children is still limited. In children, leptin is a hormone which has major influence on energy balance. Obese chidren have higher serum leptin levels compared to non- obese. The aim of this study was to compare the serum leptin levels, biochemical and antropometric parameters in obese and non-obese children and determine the relationship between serum leptin levels and obesity. 50 obese ( >30 kg/m2) and 50 non-obese (<25 kg/m2) children, aged 7-12 years, were included in this study. Measurements of heigh, weight, waist circumference of each subject were taken and body mass index (BMI) was also calculated. The levels of serum glucose, triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol were measured with routine automated laboratory methods. Serum leptin levels were determined with ELISA kits (DRG). BMI, waist circumference and weight were significantly higher in obese groups compared to non-obese groups (p<0.001). The serum leptin levels and LDL-cholesterol were significantly higher and HDL-cholesterol was found significantly lower in obese children (p<0.05). The results suggest that the elevated serum leptin level is a sensitive parameter of body composition and could be used as a biomarker for future metabolic disorders in obese children.


Özet

Obezite, dünyada giderek büyüyen ve hem çocukları hem de yetişkinleri etkileyen bir sağlık problemidir. Çocukluk çağı obezitesi insülin direnci ve metabolik sendrom gibi sağlık problemleri riskini artırmaktadır. Ayrıca çocukluk çağı obezitesi yetişkinlik döneminde obezite ve kardiovasküler hastalıkların görülme riskini de artırmaktadır. Buna rağmen çocukluk çağı obezitesinin adipositokinlerle olan ilişkisi hakkında bilgiler kısıtlıdır. Leptin çocuklarda enerji dengesini düzenleyen başlıca hormondur. Obez çocuklarda, obez olmayanlara göre serum leptin seviyesi yüksektir. Bu çalışmanın amacı obez olan ve olmayan çocuklarda serum leptin seviyesi, biyokimyasal ve antropometrik parametrelerin karşılaştırılmasıdır. Ayrıca çalışmada serum leptin seviyesi ve obezite arasında ilişkilendirme yapılması da amaçlanmıştır. Çalışmada, 7 ile 12 yaş arası 50 obez( >30 kg/m2) ve 50 obez olmayan (<25 kg/m2) çocuktan oluşan iki çalışma grubu oluşturulmuştur. Çalışmaya katılan her bireyin boyu, kilosu, kalça çevresi ölçüldü ve vücut kitle indeksi (VKİ) hesaplanmıştır. Serum glukoz, trigliserit, total kolesterol, HDL-kolesterol ve LDL kolesterol seviyeleri rutin otomatize laboratuar yöntemleri ile ölçülmüştür. Serum leptin seviyeleri ELISA kiti kullanılarak belirlenmiştir (DRG). VKİ, bel çevresi ve kilo obez kişlerde obez olmayanlara göre anlamlı derecede yüksek bulunmuştur (p<0.001). Obez çocuklarda serum leptin ve LDL-kolesterol seviyesi anlamlı derecede yüksek, HDL- kolesterol seviyesi ise anlamlı derecede düşük bulunmuştur (p<0.05). Sonuçlar gösteriyor ki serum leptin seviyesi vücut kompozisyonunun hassas bir parametresidir ve obez çocuklarda ileride ortaya çıkabilecek metabolik hastalıkları belirlemede biyomarker olarak kullanılabilir.

Serum VEGF, CD40L and NO levels in Peripheral Arterial Disease

Conference Paper Free Radical Biology and Medicine, Volume 65, Issue 1, 2013, Pages 26

Abstract

Peripheral arterial disease (PAD) is closely associated with systemic atherosclerosis and thrombosis. It has been shown that serum CD40 ligand, a transmembrane protein expressed on endothelial cells, is related to atherosclerosis from early stages to late severe thrombosis. Atherogenesis begins with endothelial dysfunction. The vascular endothelium plays a critical role in the atherothrombogenesis. The production of endothelial mediators are affected by injury of endothelium. Vascular endothelial growth factor (VEGF) is a potent endothelial cell-specific mitogen. It has been shown that VEGF plasma concentrations increased in patients with PAD. In the light of these findings, we aimed to research the levels of serum CD40L, VEGF and nitrite/nitrate levels in patients with PAD. We enrolled 106 patients with PAD and 65 age-matched healthy controls into study. The concentrations of VEGF, sCD40 ligand were determined using with ELISA method in serum samples. The final products of NO in vivo are nitrite (NO2) and nitrate (NO3). We measured serum nitrite (NO2) and nitrate(NO3) levels of the final product of NO by colorimetric method. Statistical analyses were performed using the SPSS software package, revision 15.0. p value o0.05 was considered significant. There was no difference in age between controls and patients. The mean plasma concentrations of VEGF (po0.001), sCD40L (po0.001) were higher in PAD patients than those of the controls. In contrast, the concentrations of total nitrate and nitrite were lower in patients with PAD than those of the controls (p<0.05). Increased CD40L that could play an important role in human atherosclerosis may be an indicator for PAD. Also, increased VEGF levels were compatible with the results of the other studies in PAD. However, decreased total nitrate/nitrite levels may be due to reduced synthesis and inactivation of NO in patients with PAD.


Özet

Peripheral arterial disease (PAD) is closely associated with systemic atherosclerosis and thrombosis. It has been shown that serum CD40 ligand, a transmembrane protein expressed on endothelial cells, is related to atherosclerosis from early stages to late severe thrombosis. Atherogenesis begins with endothelial dysfunction. The vascular endothelium plays a critical role in the atherothrombogenesis. The production of endothelial mediators are affected by injury of endothelium. Vascular endothelial growth factor (VEGF) is a potent endothelial cell-specific mitogen. It has been shown that VEGF plasma concentrations increased in patients with PAD. In the light of these findings, we aimed to research the levels of serum CD40L, VEGF and nitrite/nitrate levels in patients with PAD. We enrolled 106 patients with PAD and 65 age-matched healthy controls into study. The concentrations of VEGF, sCD40 ligand were determined using with ELISA method in serum samples. The final products of NO in vivo are nitrite (NO2) and nitrate (NO3). We measured serum nitrite (NO2) and nitrate(NO3) levels of the final product of NO by colorimetric method. Statistical analyses were performed using the SPSS software package, revision 15.0. p value o0.05 was considered significant. There was no difference in age between controls and patients. The mean plasma concentrations of VEGF (po0.001), sCD40L (po0.001) were higher in PAD patients than those of the controls. In contrast, the concentrations of total nitrate and nitrite were lower in patients with PAD than those of the controls (p<0.05). Increased CD40L that could play an important role in human atherosclerosis may be an indicator for PAD. Also, increased VEGF levels were compatible with the results of the other studies in PAD. However, decreased total nitrate/nitrite levels may be due to reduced synthesis and inactivation of NO in patients with PAD

Plasma Malondialdehyde and Erythrocyte Superoxide Dismutase Levels in Obesity

Conference Paper Free Radical Biology and Medicine, Volume 65, Issue 1, 2013, Pages 25

Abstract

Obesity may be a state of chronic oxidative stress. Hyperglycemia, elevated plasma lipid levels and inadequate antioxidant defenses promotes oxidative stress in obesity. The aim of this study was to determine the relationship between anthropometric parameters, plasma lipid levels and markers of oxidant/antioxidant status in obese subjects compared with non-obese subjects and its relationship with obesity-linked insulin resistance. The study included 110 obese and 90 non-obese subjects. Oxidative stress were assessed by measuring the concentration of plasma malondialdehyde (MDA). The cytoprotectic enzyme, erythrocyte superoxide dismutase (SOD) activities were measured as biological markers of antioxidant status. We also evaluated antropometric parameters and plasma lipid levels. The obese subjects had significantly higher plasma MDA levels than non-obese subjects. Erythrocyte SOD activities significantly lower in obese group compared ton non-obese group. Non-obese subjects had significantly lower HOMA-IR compared to obese subjects. Plasma MDA levels weresignificantly positively correlated with body mass index (BMI) and hip circumference in obese group. Erythrocyte SOD showed significant negative correlation with BMI in obese group. Furthermore, erythrocyte SOD were significantly negative correlated HDL-cholesterol in non-obese group. The increase in adipose tissue as a result of obesity causes a chronic inflammation which in turn may cause increases in lipid peroxidation and decreases in erythrocyte cytoprotectic. So our result suggest that obesity even in the absence of other confounding factors such as diabetes and hypertension, is an independent risk factor for lipid peroxidation and increase in oxidative stress.


Özet

Obesity may be a state of chronic oxidative stress. Hyperglycemia, elevated plasma lipid levels and inadequate antioxidant defenses promotes oxidative stress in obesity. The aim of this study was to determine the relationship between anthropometric parameters, plasma lipid levels and markers of oxidant/antioxidant status in obese subjects compared with non-obese subjects and its relationship with obesity-linked insulin resistance. The study included 110 obese and 90 non-obese subjects. Oxidative stress were assessed by measuring the concentration of plasma malondialdehyde (MDA). The cytoprotectic enzyme, erythrocyte superoxide dismutase (SOD) activities were measured as biological markers of antioxidant status. We also evaluated antropometric parameters and plasma lipid levels. The obese subjects had significantly higher plasma MDA levels than non-obese subjects. Erythrocyte SOD activities significantly lower in obese group compared ton non-obese group. Non-obese subjects had significantly lower HOMA-IR compared to obese subjects. Plasma MDA levels were significantly positively correlated with body mass index (BMI) and hip circumference in obese group. Erythrocyte SOD showed significant negative correlation with BMI in obese group. Furthermore, erythrocyte SOD were significantly negative correlated HDL-cholesterol in non-obese group. The increase in adipose tissue as a result of obesity causes a chronic inflammation which in turn may cause increases in lipid peroxidation and decreases in erythrocyte cytoprotectic. So our result suggest that obesity even in the absence of other confounding factors such as diabetes and hypertension, is an independent risk factor for lipid peroxidation and increase in oxidative stress.

Lipid Profile and Lipid Peroxidation in Beta-Thalassemia Major Patients

Conference Paper Turkish Journal of Biochemistry, Volume 38, Issue 1, 2013, Pages 279

Abstract

Aim: Beta-thalassemia major is an inherited hemoglobin disorder caused by impaired synthesis of beta globin chain. Patients with beta-thalassemia major require continuous blood transfusion which lead to iron overload. This excess iron leads to enhanced generation of reactive oxygen species and oxidative stress. Malondialdehyde (MDA), a product of lipid peroxidation is a marker of oxidative stress. The aim of this study was to determine lipid profile, levels of serum MDA and ferritin in beta-thalassemia major patients compared with healthy subjects, and to evaluate the relationships among these parameters. Materials and Methods: 53 beta-thalassemia patients and 50 healthy subjects were included in this study. Biochemical and hematological parameters were determined with routine automated laboratory methods. Serum MDA levels were determined by using thiobarbituric acid reaction substance methods. Results: Malondialdehyde levels were significantly higher in patients than in controls. Total cholesterol, HDL-cholesterol and LDL-cholesterol levels were significantly lower in patient group. Thalassemia patients showed significantly higher levels of triglycerides and ferritin. There was no significant correlation between MDA and ferritin, Total cholesterol, HDL-cholesterol, triglycerides and LDL-cholesterol levels. Conclusion: Our results suggest that iron overload promotes lipid peroxidation in beta-thalassemia patients. Low level of total cholesterol, HDL-cholesterol and LDL-cholesterol may be associated with increased cholesterol uptake by reticuloendothelial. We suggest that despite the excess lipid peroxidation in beta-thalassemia patients, low levels of cholesterol may reduce the risk of cardiovascular diseases.


Özet

Amaç: Beta-talasemi majör, beta globin zincir sentezinin bozulması sonucu hatalı hemoglobin yapımına bağlı olarak ortaya çıkan kalıtsal bir hastalıktır. Beta-talasemi majör hastaları devamlı kan transfüzyonuna ihtiyaç duymaktadır. Transfüzyon demir düzeyinde yükselmeye neden olmaktadır. Yüksek düzeydeki demir, reaktif oksijen radikallerini ve oksidatif stresi artırmaktadır. Malondialdehit (MDA), lipit peroksidasyonu sonucu oluşan bir oksidatif stres belirtecidir. Bu çalışmanın amacı beta-talasemi hastalarında lipit profili, serum MDA ve ferritin düzeylerinin belirlenerek kontrol grubu ile karşılaştırılması ve bu parametrelerin kendi içlerinde ilişkilerinin belirlenmesidir. Gereç ve Yöntem: Çalışmaya 53 beta-talasemi ve 50 sağlıklı birey dâhil edildi. Biyokimyasal ve hematolojik parametreler rutin otomatize laboratuvar metodları ile belirlendi. Serum MDA düzeyi tiyobarbitürik asit yöntemi kullanılarak belirlendi. Bulgular: Malondialdehit düzeyi kontrol grubuna göre kıyaslandığı zaman hasta grubunda istatistiksel olarak anlamlı derecede yüksek bulundu. Hasta grubunda total kolesterol, HDL- kolesterol ve LDL-kolesterol düzeyleri istatistiksel olarak anlamlı derecede düşük bulundu. Trigliserit ve ferritin seviyelerinin beta-talasemi hastalarında anlamlı derecede yüksek olduğu gözlemlendi. MDA, ferritin, total kolesterol, HDL-kolesterol, trigliserit ve LDL-kolesterol seviyeleri arasında anlamlı bir ilişki saptanmadı. Sonuç: Bulgularımız beta-talasemi hastalarında yüksek düzeydeki demirin lipit peroksidasyonunu artırdığını göstermektedir. Total kolesterol, HDL-kolesterol ve LDL-kolesterol seviyelerindeki düşüklüğün nedeni retiküloendotelyal sistem tarafından kolesterol kullanımının artmış olması ile ilişkili olabilir. Sonuç olarak beta-talasemi hastalarında lipit peroksidasyonunun artmış olmasına rağmen kolesterol seviyesinin düşük olması kardiyovasküler hastalık riskini azaltmaktadır.

ASSOCIATION OF SERUM RESISTIN LEVEL AND INSULIN RESISTANCE IN COMPARISON STUDY BETWEEN OBESE AND NON-OBESE SUBJECTS

Conference Paper De Gruyter, Volume 52, Issue 1, 2014, Pages 1378

Abstract

BACKGROUND: Resistin is an adipocyte-derived peptide that is involved in the development of obesity and insulin resistance (IR) in mice and may play a similar role in humans through mechanisms that remain unresolved. The aim of this study was to determine resistin levels in obese and non-obese subjects and evaluate their association with anthropometric parameters and insulin resistance. METHODS: The study included 110 obese and 90 non-obese subjects. Fasting glucose, HDL-cholesterol (HDL-C), triglycerides (TG), total cholesterol (TC), LDL-cholesterol (LDL-C), resistin and antropometric parameters were measured. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. RESULTS: Serum resistin levels were higher in non-obese group when compared to the obese group (p<0.001). Additionally, plasma glucose, total cholesterol, triglycerides and LDL-cholesterol levels were significantly higher in obese subjects (p<0.001) but they had significantly lower mean HDL-cholesterol (p<0.001) levels. Non-obese subjects had significantly lower HOMA-IR compared to obese subjects (p<0.001). In obese subjects, resistin levels were significantly correlated with waist circumferences (p<0.05). A significant correlation was observed between resistin level and hip circumference in non-obese group (p<0.05). Resistin was significantly correlated with HOMA-IR in obese group (p<0.05). CONCLUSIONS: Resistin levels were elevated in obese subjects and were associated with insulin resistance. These results in accordance with the previous similar studies in literature, indicate that resistin might be a possible candidate factor in obesity-related insulin resistance.


Özet

BACKGROUND: Resistin is an adipocyte-derived peptide that is involved in the development of obesity and insulin resistance (IR) in mice and may play a similar role in humans through mechanisms that remain unresolved. The aim of this study was to determine resistin levels in obese and non-obese subjects and evaluate their association with anthropometric parameters and insulin resistance. METHODS: The study included 110 obese and 90 non-obese subjects. Fasting glucose, HDL-cholesterol (HDL-C), triglycerides (TG), total cholesterol (TC), LDL-cholesterol (LDL-C), resistin and antropometric parameters were measured. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. RESULTS: Serum resistin levels were higher in non-obese group when compared to the obese group (p<0.001). Additionally, plasma glucose, total cholesterol, triglycerides and LDL-cholesterol levels were significantly higher in obese subjects (p<0.001) but they had significantly lower mean HDL-cholesterol (p<0.001) levels. Non-obese subjects had significantly lower HOMA-IR compared to obese subjects (p<0.001). In obese subjects, resistin levels were significantly correlated with waist circumferences (p<0.05). A significant correlation was observed between resistin level and hip circumference in non-obese group (p<0.05). Resistin was significantly correlated with HOMA-IR in obese group (p<0.05). CONCLUSIONS: Resistin levels were elevated in obese subjects and were associated with insulin resistance. These results in accordance with the previous similar studies in literature, indicate that resistin might be a possible candidate factor in obesity-related insulin resistance.

Association between leptin G-2548A gene polymorphism, plasma leptin levels and lipid profiles in obese women

Conference Paper XIV. ULUSAL TIBBİ BİYOLOJİ VE GENETİK KONGRESİ, Volume 1, Issue XIV, 2015, Pages 292

Abstract

Leptin is an adipocyte-secreted hormone which is present in the circulation in amounts proportional to fat mass that acts to reduce food intake and increase energy expenditure thereby regulating body weight homeostasis. Various polymorphisms are shown to be present in leptin (LEP) gene which play important roles in obesity. One of these polymorphisms is a common G-2548A leptin promoter variant. The aim of this study was to investigate the association of leptin gene G-2548A polymorphism, on obesity in association with body mass index (BMI), lipid parameters, plasma leptin levels and homeostasis model assessment of insulin resistance (HOMA-IR) in postmenopausal women. The study included 115 obese and 85 non-obese postmenopausal women. The LEP G-2548A polymorphism was determined by polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP). Plasma leptin levels, serum lipid and antropometric parameters were measured. No association was found between LEP gene G-2548 polymorphism and BMI in both study and control groups. Strikingly, subjects with the AA genotype had significantly higher serum total cholesterol (p<0.05) than GA and GG genotypes in both obese and non-obese subjects. In obese group, subjects with the AA genotypes had significantly higher triglycerides levels (p<0.05). Our results suggest that the LEP gene G-2548 polymorphism has association with triglycerides and total cholesterol levels in obese group but has no direct association with obesity in postmenopausal women.


Özet

Leptin, adipositlerden sentez edilen ve dolaşımdaki miktarı vücuttaki yağ oranı ile orantılı olarak bulunan bir hormondur. Leptin enerji harcanmasını artırarak ve yiyecek alımını kısıtlayarak vücut ağırlığı homeostazını düzenlemektedir. Leptin (LEP) geninde bulunan birçok polimorfizmin obezitede önemli rol oynadığı gösterilmiştir. G-2548A leptin promotör varyantı en yaygın görülen polimorfizmlerden bir tanesidir. Bu çalışmanın amacı, postmenopozal kadınlarda leptin gen G-2548A polimorfizmi ile vücut kitle indeksi (VKİ), lipid parametreleri, plazma leptin düzeyleri ve homeostatik model değerlendirme indeksi (HOMA-IR) arasındaki ilişkiyi belirlemektir. Çalışmaya 115 obez ve 85 obez olmayan postmenopozal kadın katılmıştır. Çalışma kapsamında açlık kan şekeri, HDL-kolesterol (HDL-K), trigliserit (TG), total kolesterol (TK), LDL-kolesterol (LDL-K), leptin, ve antropometrik parametrelerin ölçümü yapılmıştır. LEP G-2548A polimorfizmi polimeraz zincir reaksiyonu-restriksiyon uzunluk polimorfizmi (PCR-RFLP) yöntemi ile belirlenmiştir. Her iki grupta da LEP G-2548A gen polimorfizmi ile VKİ arasında ilişki saptanmamıştır. Fakat AA genotipi taşıyan obez ve obez olmayan bireylerin total kolesterol seviyelerinin GA ve GG genotipi taşıyanlara göre anlamlı derecede yüksek olduğu belirlenmiştir (p<0.05). Ayrıca obez grupta, AA genotipi taşıyan bireylerin trigliserit sevilerinin istatistiksel olarak anlamlı derecede yüksek olduğu saptanmıştır.(p <0.05). Sonuçlar gösteriyor ki LEP gen G-2548A polimorfizminin postmenopozal kadınlarda obezite ile direkt bağlantısı bulunmamaktadır. Fakat G-2548A polimorfizminin postmenopozal obez kadınlarda trigliserit ve total kolesterol seviyeleri üzerine etkisi bulunmaktadır.

Kuzey Kıbrıs Çiğ Bademlerinin Eczacılıkta Değerlendirilmesi

Conference Paper Bitkisel İlaç Hammaddeleri Toplantısı, Volume 1, Issue XXII, 2016, Pages SC12

Abstract

Akdeniz ülkelerinde yetişen Prunus amygdalus var. dulce, Kıbrıs’ın kuzeyinde de yaygındır. Drupa meyveleri çağla şeklinde iken Türkiye’dekilerden farklı, özel bir lezzeti olan Kıbrıs bademlerinin toplanması ve çekirdklerin kırılarak badem içlerinin çıkarılması imece ile yapılan bir gelenek ise de giderek azaldığından Tarım Bakanlığı badem yetiştiricilerini desteklemektedir. Kıbrıs’ta yetişen badem ağaçlarının korunması, sayılarının artırılmasına yönelik olarak bademlerin yağı ile posasının eczacılıkta değerlendirilmesi hedeflenmiştir. Güzelyurt, Yedidalga Köyü’ndeki badem ağaçlarından toplanan bademlerden TABİA Doğal Destekler ARGE Laboratuvarında süper kritik CO2 ekstraksiyon metodu ile %34.8 yağ elde edilkmiştir. Elde edilen yağ metillenerek GC-MS ile analizi yapılmıştır. Oleik asit (%77.8), linoeik asit (%13.5) ve palmitik asit (%7.4) saptanmıştır. Kuzey Kıbrıs badem yağı örneğinin Colo-320 (primer insan kolon kanseri), Colo-741 (metastatik insan kolon kanseri), MCF-7 (primer meme kanseri) ve M4A4 (metastatik meme kanseri) hücre hatlarında incelenmesi devam etmekte olup alınan ilk sonuçlar ümit vericidir.


Özet

Akdeniz ülkelerinde yetişen Prunus amygdalus var. dulce, Kıbrıs’ın kuzeyinde de yaygındır. Drupa meyveleri çağla şeklinde iken Türkiye’dekilerden farklı, özel bir lezzeti olan Kıbrıs bademlerinin toplanması ve çekirdklerin kırılarak badem içlerinin çıkarılması imece ile yapılan bir gelenek ise de giderek azaldığından Tarım Bakanlığı badem yetiştiricilerini desteklemektedir. Kıbrıs’ta yetişen badem ağaçlarının korunması, sayılarının artırılmasına yönelik olarak bademlerin yağı ile posasının eczacılıkta değerlendirilmesi hedeflenmiştir. Güzelyurt, Yedidalga Köyü’ndeki badem ağaçlarından toplanan bademlerden TABİA Doğal Destekler ARGE Laboratuvarında süper kritik CO2 ekstraksiyon metodu ile %34.8 yağ elde edilkmiştir. Elde edilen yağ metillenerek GC-MS ile analizi yapılmıştır. Oleik asit (%77.8), linoeik asit (%13.5) ve palmitik asit (%7.4) saptanmıştır. Kuzey Kıbrıs badem yağı örneğinin Colo-320 (primer insan kolon kanseri), Colo-741 (metastatik insan kolon kanseri), MCF-7 (primer meme kanseri) ve M4A4 (metastatik meme kanseri) hücre hatlarında incelenmesi devam etmekte olup alınan ilk sonuçlar ümit vericidir.

Role of leptin and adiponectin in obesity-associated oxidative stress

Conference Paper 41st Federation of European Biochemical Societies (FEBS) Congress, Volume 283, Issue S1, 2016, Pages 389

Abstract

Objective: Increased oxidative stress is one of the major characteristics of obesity and obesity-related complications. Adipokines also induce the production of reactive oxygen species and generating oxidative stress in physiological and pathological conditions of obesity. The aim of this study was to determine the association of leptin and adiponectin levels with body mass index, lipid parameters and oxidative stress biomarkers in obesity. Methods: The study included 150 obese and 120 non-obese subjects. Plasma leptin and adiponectin levels (ng/mL) were measured using commercially available enzyme-linked immunosorbent assay kits. Serum lipid, superoxide dismutase, malondialdehyde and antropometric parameters were measured. Results: Obese and non-obese subjects did not differ in age, while plasma glucose, total cholesterol, triglycerides, LDL cholesterol and leptin levels were significantly higher and mean HDL cholesterol and adiponectin levels were significantly lower in obese than non-obese subjects. The plasma leptin (p<0.001) and adiponectin (p=0.003) levels were significantly correlated with BMI in both obese and non-obese subjects. In obese subjects, leptin levels were significantly correlated with superoxide dismutase (p<0.01) and malondialdehyde (p<0.01). Strikingly, adiponectin was significantly correlated with superoxide dismutase (p=0.02) and malondialdehyde (p=0.0012) levels in obese group. Conclusion: Our results suggest that leptin and adiponectin levels are associated with defective antioxidant status and increased lipid peroxidation which may have implications in the development of obesity related health problems.


Özet

Antibacterial and antifungal effects of Corchorous olitorius L. leaf extracts on ten standard strains

Conference Paper Journal of Biotechnology, Volume 256, Issue -, 2017, Pages 100

Abstract

Corchorus olitorius L. (COL) is a plant which is widely consumed by people living in Middle East and Asia. COL is known to have medicinal properties. Phytochemicals such as alkaloids and saponins are thought to give antimicrobial effects of COL. COL leaves that were collected in North Cyprus were extracted with ethanol then filtered and concentrated to 200 ml. H2O phase was evaporated and lyophilized. Concentrated H2O phase was then extracted with dicloromethane (DCM). Micro dilution method was used for antimicrobial susceptibility test. The extracts were prepared in different concentrations. Standard bacterial and fungal microorganisms; S. aureus, E. coli, B. cereus, P. aeruginosa, S. maltophilia, C. dubliniensis, C. albicans, C. glabrata, C. parapsilosis and C. neoformans were used. The micro plates were incubated at 37 ?C for 24 h. The turbidity reading was measured by spectrophotometer. It was expected for extracts to exert antibacterial and antifungal activities however, COL extracts did not show any significant antibacterial and antifungal activities in ten standard strains. Phytochemicals which exert antibacterial/antifungal properties like alkaloids and saponins, might not have been extracted in ethanolic and DCM extracts of COL. In addition, concentrations that were used might not been the optimal amount to show antimicrobial effects.


Özet

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Isolation and Characterization of Human Foreskin Stem Cells

Conference Paper -, Volume -, Issue -, 2017, Pages 44-45

Abstract

Stem cells are unspecialized cells capable of renewing themselves, which have tremendous potential for biological and therapeutic applications. Mesenchymal stem cells can be obtained from different tissues, cultured and have potential to differentiation for somatic cells. We aimed to investigate the stem cell from the foreskin of newborns that had been discarded after circumcision. Newborn human foreskin tissue obtained following routine circumcision. The mucosa, skin and mucosa/skin part of the foreskin were collected. After enzymatic dissociation, they were cultured in DMEM supplemented with 10 % FBS, 1 % PSA 25 µg/mL amphotericin B. They were cultured for 14 days days and distribution of CD44 and CD90 were analyzed using immunocytochemical staining for characterization. After 5 days of culture time, cells were attached and proliferate in all parts of the foreskin. However, the number of mucosal cells were more than others. After subculture of the cells, proliferation rate was trigger and cells had fibroblast-homologous, spindle-formed morphology. In addition cells were positively stained both CD44 and CD90. Stem cells from newborn foreskin were isolated and characterized in our laboratory. The differentiation potential of the cells have to be analyzed. Foreskin is easily obtainable and utilizable than the other stem cell sources. This research may be open up a potential new treatment paradigm for stem cell therapy.


Özet

Kök hücreler kendilerini yenileyebilen, biyolojik ve terapötik uygulamalar için potansiyele sahip olan spesifik olmayan hücrelerdir. Mezenkimal kök hücreler, farklı dokulardan elde edilebilmektedir ve kültüre edilerek somatik hücrelere farklılaşma potansiyeline sahiptirler. Bu çalışmadaki amacımız yeni doğan sünnet derisinden kök hücrelerin izolasyonu ve karakterizasyonudur. Yeni doğan sünnet derisi rutin sünnet işlemi sonrası temin edildi. Sünnet derisi mukoza, deri ve deri/mukoza olmak üzere üç parçaya ayrıldı. Enzimatik ayrıştırma sonrası sünnet derisi parçaları 10% FBS, 1% PSA 25 µg/mL amphotericin B içeren DMEM vasatında kültüre edildi. 14 gün süreyle ile kültür işlemi sonrası elde edilen hücrelerin karakterizasyon için immünositokimyasal boyama kullanılarak CD44 ve CD90 dağılımı analiz edildi. 5 günlük kültür işlemi sonrası tüm sünnet derisi parçalarında kültür kabına tutunarak çoğalan hücreler tesbit edildi. Fakat mukozal hücrelerin sayısının diğerlerine göre daha fazla olduğu görüldü. Pasajlanma işlemi sonrası hücrelerin fibroblast homoloğu, iğ biçiminde bir morfolojiye sahip oldukları ve çoğalma hızının arttığı tesbit edildi. Ayrıca, hücrelerin CD44 ve CD90 boyanmasının pozitif olduğu belirlendi. Laboratuvarımızda yenidoğan sünnet derisinden alınan kök hücreler izole edildi ve karakterize edildi. Bu hücrelerin farklılaşma potansiyeli analiz edilmelidir. Sünnet derisi, diğer kök hücre kaynaklarına göre kolayca elde edilebilir ve kullanılabilir. Bu araştırma, kök hücre tedavisi için yeni bir tedavi paradigması açabilecek potansiyele sahiptir.

APOPTOTIC EFFECT OF CORCHORUS OLITORIUS LEAF EXTRACT IN COLON ADENOCARCINOMA CELL LINES

Conference Paper The Third Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-3), Volume -, Issue -, 2017, Pages -

Abstract

Objective: The objective of the study was to determine antiapoptotic activities of Corchorus olitorius L. (Co) leaf extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Material and Methods: Dry, powdered Co leaves (100 g) that were collected in July 2016 were extracted with ethanol then filtered and concentrated to 200 ml. H2O phase was evaporated and lyophilized to yield 14,8 g of crude extract. Different concentrations of Co extract were incubated for 24 and 48 h on Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured with MTT assays. Apoptotic activities of Co were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and Fas ligand (FasL). TUNEL assay was used to detect DNA fragmentation in both cell lines. Results: While the number of TUNEL positive cells were similar in both cell lines after incubation with extract, caspase-3 expression was higher in Colo-320 cells than Colo-741 cells after 24 h incubation. On the other hand, immunoreactivity of cytochrome-c and FasL were higher in Colo-741 cells. Caspase-3 expression was lower in both Colo-320 and Colo-741 cells when compared with cytochrome-c and FasL expression. Discussion/Conclusion: H2O phase extract of Co leaf had triggered apoptotic pathway in both cell types, but, Colo-741 cells were affected more than Colo-320 cells. This result suggested that, initiation of apoptotic pathway starts after 24 h and could not be terminated in 24 h. Therefore, longer incubation of Co extract in colon adenocarcinoma cell line might be more effective for treatment.


Özet

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Apoptotic Effect of Corchorus olitorius Leaf Extract in Colon Adenocarcinoma Cell Lines

Conference Paper The Third International Mediterranean Symposium on Medicinal and Aromatic Plants, Volume ISBN: 978-605-61261-2-3, Issue 3, 2017, Pages 140

Abstract

Objective: The objective of the study was to determine antiapoptotic activities of Corchorus olitorius L. (Co) leaf extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Material and Methods: Dry, powdered Co leaves (100 g) that were collected in July 2016 were extracted with ethanol then filtered and concentrated to 200 ml. H2O phase was evaporated and lyophilized to yield 14,8 g of crude extract. Different concentrations of Co extract were incubated for 24 and 48 h on Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured with MTT assays. Antiapoptotic activities of Co were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and Fas ligand (FasL). TUNEL assay was used to detect DNA fragmentation in both cell lines. Results: While the number of TUNEL positive cells were similar in both cell lines after incubation with extract, caspase-3 expression was higher in Colo-320 cells than Colo-741 cells after 24 h incubation. On the other hand, immunoreactivity of cytochrome-c and FasL were higher in Colo-741 cells. Caspase-3 expression was lower in both Colo-320 and Colo-741 cells when compared with cytochrome-c and FasL expression. Discussion/Conclusion: H2O phase extract of Co leaf had triggered apoptotic pathway in both cell types, but, Colo-741 cells were affected more than Colo-320 cells. This result suggested that, initiation of apoptotic pathway starts after 24 h and could not be terminated in 24 h. Therefore, longer incubation of Co extract in colonadenocarcinoma cell line might be more effective for treatment.


Özet

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http://www.nobelmedicus.com/en/Article.aspx?m=518

General Publication RELATIONSHIP BETWEEN LEPTIN-ADIPONECTIN RATIO AND INSULIN RESISTANCE IN OBESE SUBJECTS, 2016

Abstract


Özet

https://www.infona.pl/resource/bwmeta1.element.elsevier-398ad78c-a24b-3c0a-b69a-040000c31d43

General Publication Serum VEGF, CD40L and NO levels in Peripheral Arterial Disease, 2016

Abstract


Özet

http://www.sciencedirect.com/science/article/pii/S0378111915005478

General Publication Association of the Ala16Val MnSOD gene polymorphism with plasma leptin levels and oxidative stress biomarkers in obese patients, 2016

Abstract


Özet

http://scholar.google.com/scholar?cluster=16215352956056632633&hl=en&oi=scholarr

General Publication PO5-138 ENDOTHELIAL NITRIC OXIDE SYNTHASE G894T GENE POLYMORPHISM, BLOOD PRESSURE AND HYPERTENSION IN THE TURKISH ADULT RISK FACTOR STUDY, 2016

Abstract


Özet

http://www.sciencedirect.com/science/article/pii/S0378111913010184

General Publication Association of leptin receptor gene Q223R polymorphism on lipid profiles in comparison study between obese and non-obese subjects, 2016

Abstract


Özet

http://www.degruyter.com/view/j/tjb.2016.41.issue-1/tjb-2016-0001/tjb-2016-0001.xml

General Publication Association between leptin G-2548A gene polymorphism, plasma leptin levels and lipid profiles in Turkish Cypriot obese subjects/Kıbrıslı Türk obez kişilerde leptin G-2548A gen polimorfizmi ile plazma leptin seviyeleri ve lipid profili arasındaki ilişki, 2016

Abstract


Özet

http://scholar.google.com/scholar?cluster=11093742845326420944&hl=en&oi=scholarr

General Publication OBEZ KİŞİLERDE Q223R POLİMORFİZMİ İLE LEPTİN, ADİPONEKTİN, RESİSTİN VE İNSÜLİN DİRENCİ ARASINDAKİ İLİŞKİNİN İNCELENMESİ, 2016

Abstract


Özet

https://www.infona.pl/resource/bwmeta1.element.elsevier-7a8db2ee-8515-3842-b4d2-d23fb3407dde

General Publication Plasma Malondialdehyde and Erythrocyte Superoxide Dismutase Levels in Obesity, 2016

Abstract


Özet

http://www.turkiyeklinikleri.com/article/en-serum-total-antioxidant-status-lipid-profile-malondialdehyde-and-erythrocyte-superoxide-dismutase-levels-in-hashimoto-thyroiditis-patients-treated-with-levothyroxine-63049.html

General Publication Serum total antioxidant status, lipid profile, malondialdehyde and erythrocyte superoxide dismutase levels in Hashimoto thyroiditis patients treated with levothyroxine, 2016

Abstract


Özet

Serum Total Antioxidant Status, Lipid Profile,Malondialdehyde and Erythrocyte Superoxide Dismutase Levels in Hashimoto Thyroiditis

Original Article Turkiye Klinikleri J Med Sci, Volume 5, Issue 32, 2012, Pages 1241-1246

Abstract

ABS TRACT Ob jec ti ve: To compare the serum level of total antioxidant status (TAS), malondialdehyde (MDA) and erythrocyte superoxide dismutase (SOD) levels in Hashimoto thyroiditis patients treated with levothyroxine and healthy control subjects and to assess possible correlations of these parameters with biochemical parameters such as glucose and lipid profile. Ma te ri al and Met hods: SOD, TAS, MDA, fasting glucose, lipid profile, thyroid hormones and body mass index (BMI) were measured in 30 Hashimoto thyroiditis patients treated with levothyroxine and 30 healthy control subjects. Re sults: Body Mass Index was increased in Hashimoto thyroiditis patients compared to control subjects. The biochemical parameters such as glucose, triglycerides, total cholesterol and low-density lipoprotein cholesterol (LDL-C) were higher and high-density lipoprotein cholesterol (HDL-C) was lower in the patient group than in control patients. TAS and MDA levels were significantly higher and erythrocyte SOD levels were lower in the patient group compared to the control group. Conc lu si on: Our study showed that lipid peroxidation was increased in hypothyroid patients compared to healthy controls, even though they were treated with levothyroxine. Erytrocyte SOD levels were lower and TAS was higher in the patient group compared to the control group. Thyroiditis patients treated with levothyroxine had normal thyroid homone ranges but treatment did not seem to improve dyslipidemia and oxidative stres. In conclusion, although the hypothyroid patients had normal thyroid hormone levels due to levothyroxine treatment, oxidative stress parameters seemed to contiue to increase. Thus, we suggest that antioxidant treatment may be continued even after the achievement of euthyroidism.


Özet

Association of leptin receptor gene Q223R polymorphism on lipid profiles in comparison study between obese and non-obese subjects

Original Article GENE, Volume 529, Issue 1, 2013, Pages 16-20

Abstract

Objectives: Leptin is a hormone secreted from adipocytes. It regulates metabolism and energy homeostasis through the leptin receptor (LEPR) which is localized centrally in hypothalamus as well as in peripheral tissues. The aim of this study was to investigate the association of leptin receptor gene Q223R polymorphism on obesity in associationwith bodymass index (BMI), lipid parameters, plasma leptin levels and homeostasis model assessment of insulin resistance (HOMA-IR). Design and methods: The study included 110 obese and 90 non-obese subjects. The LEPR Q223R polymorphism was determined by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). Plasma leptin levels, serum lipid and antropometric parameters were measured. Results: No association was found between LEPR gene Q223R polymorphism and BMI in both study and control groups. Strikingly study groupwith non-obese subjects andwith the RR genotype (homozygousmutant) had significantly higher serum total cholesterol (p b 0.001) and low density lipoprotein cholesterol (LDL-cholesterol) levels (p b 0.05) than QR (heterozygous) and QQ (wild type) genotypes. In obese group, subjectswith the RR genotypes had significantly higher triglycerides (p b 0.05) levels, waist (p b 0.05) and hip circumferences (p b 0.001) than the QQ and QR genotypes. Conclusions: Our results suggest that the LEPR gene Q223R polymorphism has an association with waist and hip circumferences in obese group but no direct association with obesity although there is a significant influence on lipid profile both in obese and non-obese subjects.


Özet

RELATIONSHIP BETWEEN LEPTIN-ADIPONECTIN RATIO AND INSULIN RESISTANCE IN OBESE SUBJECTS

Original Article Nobel Medicus, Volume 11, Issue 3, 2015, Pages 10-16

Abstract

ABSTRACT Objective: Leptin and adiponectin are important hormones secreted from adipocytes that are involved in the regulation of energy homeostasis and might contribute to the link between obesity and insulin resistance. The aim of this study was to determine the relationships among various adipokine measures (leptin, adiponectin, and the ratio of leptin to adiponectin (L/A)), insulin resistance, and obesity. Material and Method: The study included 110 obese and 90 non-obese subjects. Fasting glucose, insulin, HDL cholesterol (HDL-C), triglycerides (TG), total cholesterol (TC), LDL cholesterol (LDL-C), leptin, adiponectin and anthropometric parameters were measured. Each subject’s homeostasis model assessment of insulin resistance (HOMA-IR) index and leptin to adiponectin ratio (L/A) were calculated. Results: The L/A ratio was significantly correlated with HOMA-IR in both obese (r=0.25, p<0.01) and non-obese (r=0.68, p<0.01) subjects. In obese subjects, leptin and the L/A ratio were significantly correlated with body mass index (BMI) (p<0.01) and waist (p<0.01) and hip (p<0.01) circumferences and adiponectin was significantly correlated with HOMA-IR (r=-0.68, p<0.01). Best cut off value of L/A for BMI was found to be 0.78, sensitivity was 93.6% and specificity was 81.1% according to receiver operating characteristic (ROC) analysis. Conclusion: The results suggest that the L/A ratio might be a more sensitive and reliable indicator of insulin resistance in obese subjects than adiponectin or leptin concentrations.


Özet

Association of the Ala16Val MnSOD gene polymorphism with plasma leptin levels and oxidative stress biomarkers in obese patients

Original Article GENE, Volume 568, Issue 1, 2015, Pages 35-39

Abstract

Chronic oxidative stress is amajor characteristic of obesity. Manganese superoxide dismutase (MnSOD) is an antioxidant enzyme known to be present within mitochondria and is considered a main defense against oxidative stress. The aimof this study was to investigate the association between the MnSOD gene Ala16Val polymorphism in obesity in terms of body mass index (BMI), lipid parameters, plasma leptin levels, homeostasis model assessment of insulin resistance (HOMA-IR), and oxidative stress biomarkers. The study included 150 obese and 120 non-obese subjects. The MnSOD Ala16Val polymorphism was determined by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Plasma leptin levels, serum lipid, superoxide dismutase (SOD), malondialdehyde (MDA), and anthropometric parameters were measured. No association was found between the MnSOD gene Ala16Val polymorphism and BMI in the study or control group. Strikingly, in the study group, obese subjects with the VV genotype had significantly higher plasma leptin levels (p b 0.001) than those with the AA and AV genotypes. Serum total cholesterol (p b 0.01) and MDA (p b 0.001) levels were significantly higher in subjects with the VV genotype for MnSOD in the obese and non-obese groups. In the obese group, subjects with the VV genotype had significantly lower SOD (p b 0.001) activity than the AA and AV genotypes. Our results suggest that the MnSOD gene polymorphism was associated with leptin levels and superoxide dismutase activity in the obese group but had no direct association with obesity. Moreover, the Ala16Val polymorphism has a significant effect on lipid profiles and MDA levels in both obese and non-obese subjects.


Özet

Association between leptin G-2548A gene polymorphism, plasma leptin levels and lipid profiles in Turkish Cypriot obese subjects

Original Article Turkish Journal of Biochemistry , Volume 41, Issue 1, 2016, Pages 1-8

Abstract

Objective: Leptin (LEP) is a metabolic and neuroendocrine hormone which is present in the circulation in amounts proportional to fat mass that acts to reduce food intake and increase energy expenditure thereby regulating body weight homeostasis. Various polymorphisms are shown to be present in LEP gene which play important roles in obesity and obesity-related metabolic biomarkers. The aim of this study was to investigate the association of one of these polymorphisms, leptin gene G-2548A polymorphism, on obesity in association with body mass index (BMI), lipid parameters, plasma leptin levels and homeostasis model assessment of insulin resistance (HOMA-IR). Materials and Methods: The study included 110 obese and 90 non-obese subjects. The LEP G-2548A polymorphism was determined by polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP). Plasma leptin levels, serum lipid and antropometric parameters were measured. Results: No association was found between LEP gene G-2548A polymorphism and BMI in both study and control groups. Strikingly study group with obese subjects and with the AA genotype had significantly higher serum total cholesterol (p<0.05) than GA and GG genotypes. In obese group, subjects with the AA genotypes had significantly higher leptin (p<0.05) levels than the GG and GA genotypes. Conclusion: Our results suggest that the LEP gene G-2548A polymorphism may not be considered as a genetic risk factor for obesity in Turkish Cypriot population. However, the G-2548A polymorphism appear to be important in regulating leptin and total cholesterol levels in obese group through leptin gene expression and signaling.


Özet

EFFECT OF THE Pro12Ala POLYMORPHISM OF THE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR ?2 GENE ON LIPID PROFILE AND ADIPOKINES LEVELS IN OBESE SUBJECTS

Original Article Balkan Journal of Medical Genetics, Volume 20, Issue 1, 2017, Pages 71-80

Abstract

Peroxisome proliferator-activated receptor ? (PPAR?) is a key regulator of metabolism, adipokines production and secretion. The aim of this study was to investigate the association between the PPAR?2 gene Pro12Ala polymorphism in obesity in terms of body mass index (BMI), lipid parameters, homeostasis model assessment of insulin resistance (HOMA-IR), serum lipid, leptin, adiponectin, resistin and chemerin levels. The study included 160 obese and 140 non obese subjects. The Pro12Ala polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum lipid, leptin, adiponectin, resistin and chemerin levels were measured. No association was found between the Pro12Ala polymorphism and BMI. Strikingly, in the study group, obese subjects with the AA genotype had significantly higher triglycerides (p= 0.046) and resistin (p <0.001) levels than those with the wild-type PP and heterozygous PA genotypes. Serum leptin and chemerin levels were significantly associated with Pro-12Ala poymorphism in the obese and non obese groups (p<0.01). In the obese group, subjects with the homozygous AA genotype had significantly lower adiponectin (p = 0.010) activity than the PP genotype. Our results suggest that the PPAR?2 gene Pro12Ala polymorphism has no direct association with obesity but does have significant influences on lipid profiles and adipokines levels.


Özet

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Fatty acid composition and anticancer activity in colon carcinoma cell lines of Prunus dulcis seed oil

Original Article Pharmaceutical Biology, Volume 55, Issue 1, 2017, Pages 1239-1248

Abstract

ABSTRACT Context: Almond oil is used in traditional and complementary therapies for its numerous health benefits due to high unsaturated fatty acids content. Objectives: This study investigated the composition and in vitro anticancer activity of almond oil from Northern Cyprus and compared with almond oil from Turkey. Materials and methods: Almond oil from Northern Cyprus was obtained by supercritical CO2 extraction and analyzed by GC-MS. Almond oil of Turkey was provided from Turkish pharmacies. Different concentrations of almond oils were incubated for 24 and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of almond oils were investigated by immunocytochemistry using antibodies directed against to BMP-2, b-catenin, Ki-67, LGR-5 and Jagged 1. Results: Oleic acid (77.8%; 75.3%), linoleic acid (13.5%; 15.8%), palmitic acid (7.4%; 6.3%), were determined as the major compounds of almond oil from Northern Cyprus and Turkey, respectively. In the MTT assay, both almond oils were found to be active against Colo-320 and Colo-741 cells with 1:1 dilution for both 24 h and 48 h. As a result of immunohistochemical staining, while both almond oils exhibited significant antiproliferative and anticancer activity, these activities were more similar in Colo-320 cells which were treated with Northern Cyprus almond oil. Discussion and conclusion: Almond oil from Northern Cyprus and Turkey may have anticancer and antiproliferative effects on colon cancer cells through molecular signalling pathways and, thus, they could be potential novel therapeutic agents.


Özet

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