Near East University, Pharmaceutical Botany
12 Haziran 1988'de Üsküdar'da doğdu. Ailenin tek çoçuğudur. Liseyi Özel Kocaeli Kolejinde okudu. Lise öğreniminin ardından 2006 yılında Hacettepe Üniversitesi Fen Fakültesi Biyoloji bölümünü kazandı ve yüksek öğrenimini 2011 yılında orada tamamladı. Aynı yıl Yakın Doğu Üniversitesi Eczacılık Fakültesi Farmasötik Botanik Anabilim Dalında yüksek lisans öğrenimine başladı ve 2014 yılında öğrenimini tamamladı. Aynı Anabilim dalında doktora öğrenimi devam etmektedir (2014- ). Yüksek lisans öğrenimine başladığı tarihten itibaren araştırma görevlisi olarak görev almaktadır. Aynı anabilim dalında araştırma görevlisi olan Azmi HANOĞLU ile evlidir.
Near East University, Pharmaceutical Botany
Yakın Doğu Üniversitesi
Çalış, İ., Özkum Yavuz, D., Hanoğlu, A., Yiğit Hanoğlu, D., Demirci,B., Başer, K.H.C., Kıbrıs'ın Kuzey'inde Yetişen Üç Phlomis Türü Üzerine Fitokimyasal ve Biyolojik Araştırmalar, SAG-1-2016-043, Bilimsel Araştırmalar Projeleri (BAP)- 2016.
Meriçli, F., Meriçli, A.H., Hanoğlu Yiğit, D., Vatansever, S., Becer, E., Kıbrıs Babutsa (Opuntia ficus indica) Yeşil Yaprak-Gövde Ekstresinin Eczacılık Açısından Değerlendirilmesi, SAG-2017-01-006, Bilimsel Araştırmalar Projeleri (BAP)-2017.
Meriçli, AH., Meriçli, F., Özkum Yavuz, D., Hanoğlu, A., Hanoğlu Yiğit, D., Kuzey Kıbrıs’ta Yetişen Colchicum pusillum Türünün Alkaloitleri, SAG-2-2016-030, Bilimsel Araştırmalar Projeleri (BAP)- 2016.
Özkum Yavuz,D., Serakıncı, N., Yiğit Hanoğlu, D., Hanoğlu, A., Mavis, M., Demirci, B., Başer, K.H.C., KKTCde Doğal Olarak Yetişen Lamiaceae Familyasına ait Bazı Türlerin Uçucu Yağlarının GC-MS Analizleri ve Biyolojik ve Antikanser Aktivitelerinin Belirlenmesi, SAG-1-2016-040, Bilimsel Araştırmalar Projeleri (BAP)- 2016
Özkum Yavuz, D, H.K. Süer, Hanoğlu, A. , Yiğit Hanoğlu, D., Güvenir, M., K.K.T.C.’de Doğal Olarak Yetişen Thymus capitatus (tülümbe/kekik) Bitkisinin Uçucu Yağının Disk Difüzyon Yöntemiyle Antiülserojenik aktivitesinin Saptanması, 2015.
Özkum Yavuz, D., Yiğit Hanoğlu, D., Hanoğlu, A., Demirci,B., Başer, K.H.C., Çalış, İ., Kıbrıs'ın Kuzeyinde Doğal Olarak Yetişen Endemik Sideritis cypria POST Bitkisinin Fitokimyasal ve Biyolojik Aktivite Çalışmaları, SAG-1-2016-044, Bilimsel Araştırmalar Projeleri (BAP)- 2016.
Context: Almond oil is used in traditional and complementary therapies for its numerous health benefits due to high unsaturated fatty acids content. Objectives: This study investigated the composition and in vitro anticancer activity of almond oil from Northern Cyprus and compared with almond oil from Turkey. Materials and methods: Almond oil from Northern Cyprus was obtained by supercritical CO2 extraction and analyzed by GC-MS. Almond oil of Turkey was provided from Turkish pharmacies. Different concentrations of almond oils were incubated for 24 and 48?h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of almond oils were investigated by immunocytochemistry using antibodies directed against to BMP-2, ß-catenin, Ki-67, LGR-5 and Jagged 1. Results: Oleic acid (77.8%; 75.3%), linoleic acid (13.5%; 15.8%), palmitic acid (7.4%; 6.3%), were determined as the major compounds of almond oil from Northern Cyprus and Turkey, respectively. In the MTT assay, both almond oils were found to be active against Colo-320 and Colo-741 cells with 1:1 dilution for both 24?h and 48?h. As a result of immunohistochemical staining, while both almond oils exhibited significant antiproliferative and anticancer activity, these activities were more similar in Colo-320 cells which were treated with Northern Cyprus almond oil. Discussion and conclusion: Almond oil from Northern Cyprus and Turkey may have anticancer and antiproliferative effects on colon cancer cells through molecular signalling pathways and, thus, they could be potential novel therapeutic agents.
In this study, chemical composition and antimicrobial activity of the essential oil of Sideritis cypria, which is an endemic taxon in Northern Cyprus, were investigated. The essential oil obtained by hydrodistillation were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In general, the GC and GC-MS analysis illustrated that fifty compounds were identified representing 96.9% of the oil. Major components were epi-cubebol (11.9%), trans-piperitol (8.9%) and pinene (8.1%), respectively. The antimicrobial activity of the essential oil was tested by disc diffusion method against Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC 10876), Staphylococcus aureus (ATCC 25923) bacteria and Candida albicans (ATCC 90028) yeast. The results showed that the inhibition zone of S. cypria essential oil against C. albicans at maximum dose (10 µl) was higher than positive control Nystatin. The oil showed similar inhibitory zone to that of the positive control (TMP-SMX) against B. cereus at maximum dosage 10 µl. In addition, the inhibitory effect of the oil against E. coli measured only at the 10 µl as 10.33 ± 0.58. Furthermore, the inhibition zones against S. aureus was ranged from 9.00 ± 2.00 to 24.00 ± 1.73 while P. aeruginosa was found to be resistant in all doses.
Wild growing Thymus capitatus (L.) Hoff.m. & Link (Lamiaceae) was collected from three different regions of T.R.N.C. from eastern (Yildirim-Tc1), northern (Girne/Bogaz-Tc2) and western (Yedidalga-Tc3) sides. The plant materials were subjected to hydrodistillation method in order to obtain essential oils.The composition analysis were performed by GC-FID and GC-MS systems, simultaneously. The results revealed that major components of three regions were same but the percentages showed variations moderately. Altogether, number of the compounds and the accounted percentages for Tc1, Tc2 and Tc3 were 24–99.7 %, 29–99.9 % and 23–99.8 %, respectively. Major components were thymol (46–62 %) followed by p-cymene (11–17 %), ?-terpinene (4–10 %) and carvacrol (6–7 %). Thymus capitatus is a potential thymol source especially collected from Yedidalga Tc3 location with regard to the results of analysis.
Plant derived natural products can provide promising anti-cancer activity and can support the development of novel anti-cancer treatment strategies as cytotoxic activity towards cancer cells was observed in the recent studies in which different human cancer cell lines were exposed to plant essential oils. In our study, thyme (Thymus capitatus) essential oil analyzed by GC and GC/MS showed high content of thymol, p-cymene, carcacrol and ?-terpinene representing 62.3%, 10.9%, 6.7% and 5.1% of total oil respectively. It was tested for its potential therapeutic role by using neoplastic transformed human telomerase reverse transcriptase (hTERT) immortalized mesenchymal stem cells (hMSC- telo1) and a tumorigenic cell line; human telomerase reverse transcriptase immortalized mesenchymal stem cells which are irradiated with 2.5 Gy of gamma-rays. The hMSC- telo1 and irradiated hMSC- telo1cells were exposed to increasing concentrations of thyme essential oil in order to investigate the effects of different concentrations of thyme essential oil on the hMSC- telo1 and irradiated hMSC- telo1cells and to identify any cellular response of thyme essential oil towards these cell lines. The hMSC- telo1and irradiated hMSC- telo1cells were treated with 0.5% (v/v), 1% (v/v) and 2% (v/v) thyme essential oil. Cellular response of hMSC- telo1 and irradiated hMSC- telo1cells to thyme essential oil was evaluated for all three concentrations at different time points (0 h, 2 h, 4 h, 24 h, 72 h and 120 h) by investigating proliferation rate, viability and morphology. Our results demonstrated that there was significant effect of all three concentrations used on hMSC- telo1and irradiated hMSC- telo1cells; particularly the effect was higher on the cells treated with 0.5% (v/v) thyme essential oil. Furthermore, when cellular response at different time points were compared, it was shown that exposure time to thyme essential oil is highly important for cellular viability.